图1. 南瓜子叶外植体的离体再生体系建立
(A) MS培养基中萌发3天的种子(Bar=1 cm); (B)-(E) 在不定芽诱导培养基(含激素的MS培养基)中分别培养3天、10天、20天和4周时的外植体, 其中, (D) 在切口处已见愈伤组织产生; (E) 已见芽原基((B)-(C) Bars=2 cm; (D) Bar=22.5 mm; (E) Bar=15 mm); (F) 外植体接种7周产生的不定丛生芽(Bar=15 mm); (G) 伸长芽(Bar=1 cm); (H) 再生植株(Bar=2 cm); (I), (J) 炼苗移栽((I) Bar=35 mm; (J) Bar=40 mm)。

Figure 1. Establishment of plant regeneration of cotyledons of pumpkin
(A) Cultivation for 3 days: seed germination in MS (Bar=1 cm); (B)-(E) Cultivation of explants in shoot regeneration medium (SRM, MS with hormone) for 3 days, 10 days, 20 days and 4 weeks, respectively, (D) Callus induction from explants; (E) Differentiation of bud primordium from callus (Bars=2 cm in (B) and (C), Bar=22.5 mm in (D), 15 mm in (E)); (F) Cultivation for 7 weeks: shoots induction in SRM (Bar=15 mm); (G) Elongating of shoots (Bar=1 cm); (H) Regenerated plant (Bar=2 cm); (I), (J) Exercising and transplanting((I) Bar=35 mm; (J) Bar=40 mm).