南瓜高效再生体系的建立

郭佳,李衍素,贺超兴,闫妍,于贤昌

Establishing a High-efficiency Regeneration System in Pumpkin (Cucurbita moschata)

Jia Guo,Yansu Li,Chaoxing He,Yan Yan,Xianchang Yu

表5 不同浓度6-BA与ABA配比对南瓜芽再生的影响

Table 5 Effects of different concentrations of 6-BA and ABA on the bud induction of pumpkin

Treat- ments 6-BA (mg∙L-1) ABA (mg∙L-1) Number of explants Number of explants with adventitious shoots Number of shoots Average number of induced shoots Rate of plant regeneration (%) T1 0.5 0.5 60 48.64 148.36 3.05 81.07 T2 0.5 1.0 60 44.53 95.74 2.15 74.22 T3 0.5 1.5 60 46.31 135.24 2.92 77.19 T4 0.5 2.0 60 36.35 68.70 1.89 60.58 T5 0.5 2.5 60 34.88 60.00 1.72 58.14 T6 1.0 0.5 60 53.87 228.00 4.21 90.26 T7 1.0 1.0 60 51.52 170.53 3.31 85.86 T8 1.0 1.5 60 52.39 193.33 3.69 87.32 T9 1.0 2.0 60 50.55 92.00 1.82 84.25 T10 1.0 2.5 60 37.36 54.17 1.45 62.26 T11 1.5 0.5 60 51.50 183.33 3.56 85.83 T12 1.5 1.0 60 52.80 192.20 3.64 88.01 T13 1.5 1.5 60 36.28 46.07 1.27 60.46 T14 1.5 2.0 60 41.36 64.53 1.56 68.94 T15 1.5 2.5 60 26.59 32.17 1.21 44.32 T16 2.0 0.5 60 43.36 114.47 2.64 72.27 T17 2.0 1.0 60 40.25 80.91 2.01 67.09 T18 2.0 1.5 60 35.53 57.21 1.61 59.22 T19 2.0 2.0 60 31.78 39.73 1.25 52.97 T20 2.0 2.5 60 14.29 15.00 1.05 23.81 T21 2.5 0.5 60 35.98 63.33 1.76 59.97 T22 2.5 1.0 60 34.11 50.82 1.49 56.85 T23 2.5 1.5 60 29.30 35.45 1.21 48.84 T24 2.5 2.0 60 30.97 38.40 1.24 51.61 T25 2.5 2.5 60 28.36 33.75 1.19 47.27