图4. 拟南芥减数分裂重组抑制突变体正向遗传学筛选示意图
突变体正向遗传学的筛选建立在拟南芥野生型(WT)遗传背景基础上, 利用花粉荧光标记系FTL-I2b半合子荧光标记(I2b/++)进行高通量重组率检测, 筛选获得重组率提高的突变体。图中FTL-I2b中的DsRed和eYFP荧光标记分别由R和G表示。m代表基因的显性或隐性突变。RF: 重组率

Figure 4. A schematic illustration of Arabidopsis meiotic anti-CO mutants forward genetics screen
The forward genetics mutants screen was used the FTL-I2b containing linked hemizygous fluorescent reporters (I2b/++) on the genetic background of Arabidopsis wild type (WT). High-throughput detecting the recombination frequency by flow cytometry could identify the mutants with increased recombination rate. The DsRed and eYFP fluorescent reporters of FTL-I2b are indicated by R and G, respectively. The m refers to a dominant or recessive mutation. RF: Recombination frequency