图2. 拟南芥FTL-I2b半合子荧光标记(I2b/++)
(A) FTL-I2b与Col杂交示意图, F₁代经过杂交后具有半合子荧光标记, 通过减数分裂形成重组和未重组的配子; (B) 杂交F₁代花粉半合子荧光标记显微图, 图中黄色花粉(GR)为未重组配子, 单红色(R)和单绿色(G)花粉为重组配子(Bars=50 μm); (C) 利用流式细胞仪统计花粉中荧光标记可计算该标记区间I2b的重组率, 计算公式为重组率(RF)=(G+R)/(2GR+G+R)×100。

Figure 2. The Arabidopsis hemizygous fluorescent markers (I2b/++) of FTL-I2b
(A) A schematic illustration of FTL-I2b cross with Col, the hybrid F₁ contains a hemizygous fluorescent markers after hybridization, the recombined and unrecombined gametes are formed through meiosis; (B) Fluorescence micrograph of pollen in hybrid F₁ generation, in which yellow pollen (GR) is unrecombined gamete, and monochromatic red (R) and green (G) pollen is recombined gametes (Bars=50 μm); (C) The recombination frequency (RF) of I2b interval can be calculated by counting the fluorescent marker pattern in the gametes via flow cytometry, the formula calculating recombination frequency=(G+R)/(2GR+G+R)×100.