发根农杆菌介导的菠菜毛状根遗传转化体系的建立
徐悦1,曹英萍2,王玉2,付春祥2,*(
),戴绍军1,3,*()
),戴绍军1,3,*()
Agrobacterium rhizogenes-mediated Transformation System of Spinacia oleracea
Yue Xu1,Yingping Cao2,Yu Wang2,Chunxiang Fu2,*(),Shaojun Dai1,3,*()
),Shaojun Dai1,3,*()
图6. 菠菜毛状根rolB基因和GFP基因的PCR检测和荧光观察
(A) 在自然光下的转基因毛状根; (B) 在蓝色激发光下观察的转基因毛状根; (C) PCR检测转rolB (上)、VirG (中)和GFP (下)基因(1: 2 kb DNA marker; 2-11: 不同转基因毛状根株系; 12: LBA9402; 13: 野生型根; 14: 去离子水)。(A), (B) Bars=20 μm
Figure 6. PCR analysis and GFP green fluorescence assay of the rolB gene and GFP gene in spinach transgenic hairy roots
(A) Transgenic hairy root under the natural light; (B) Transgenic hairy root under the blue exciting light; (C) Genomic PCR analysis of rolB (up) and virG (middle) and GFP (down) genes in transgenic hairy roots (Lane 1: 2 kb DNA marker; Lane 2-11: Different of transgenic hairy roots lines; Lane 12: LBA9402 (GFP); Lane 13: The wild type root; Lane 14: Milli Q water). (A), (B) Bars=20 μm
