图5. 菠菜毛状根再生体系
(A) 毛状根在愈伤诱导培养基上产生愈伤组织; (B) 毛状根诱导的愈伤组织在分化培养基中生长并产生不定芽; (C) 毛状根经由愈伤组织诱导分化产生再生植株; (D) 野生型种子萌发的植株; (E) PCR检测rolB (上)和VirG (下)基因(1: 2 kb DNA marker; 2-4: 不同毛状根再生株系; 5: LBA9402; 6: 野生型; 7: 去离子水)。(A)-(D) Bars=4 cm

Figure 5. Regeneration system of spinach hairy roots
(A) Calli produced from hairy roots on callus induction medium; (B) Calli induced from hairy roots and produced adventitious buds on the regeneration medium; (C) The plantlets regenerated from the calli produced from hairy roots; (D) The wild type plantlets; (E) Genomic PCR analysis of rolB (up) and virG (down) genes in the plantlets regenerated from the calli produced from hairy roots (Lane 1: 2 kb DNA marker; Lane 2-4: Different hairy roots plantlets regenerated from the calli produced; Lane 5: LBA9402; Lane 6: The wild type plantlets; Lane 7: Mili Q water). (A)-(D) Bars=4 cm