发根农杆菌介导的菠菜毛状根遗传转化体系的建立
徐悦1,曹英萍2,王玉2,付春祥2,*(),戴绍军1,3,*()
Agrobacterium rhizogenes-mediated Transformation System of Spinacia oleracea
Yue Xu1,Yingping Cao2,Yu Wang2,Chunxiang Fu2,*(),Shaojun Dai1,3,*()

图3. 菠菜毛状根诱导及其形态特点
(A) 发根农杆菌(LBA9402)侵染之后在1个位点产生多条毛状根; (B) 叶柄维管束部位产生的毛状根; (C) 菠菜叶脉部位产生的毛状根; (D) 茎的维管束部位产生的毛状根; (E) PCR分析毛状根中rolB (上)和VirG (下)基因(1: 2 kb DNA marker; 2-11: 不同毛状根再生植株; 12: LBA9402; 13: 非转化对照根; 14: 去离子水); (F) LBA9402诱导产生的毛状根; (G) 野生型根。(A)-(D), (F), (G) Bars=20 μm

Figure 3. The induction and morphological characteristics of spinach hairy roots
(A) After Agrobacterium rhizogenes LBA9402 infection, multiple hairy roots were produced at one site; (B) The induction of hairy roots around the vascular bundle of the petiole; (C) The induction of hairy roots in veins; (D) The induction of hairy roots around the vascular bundle of stem; (E) Genomic PCR analysis of rolB (up) and virG (down) genes in hairy roots (Lane 1: 2 kb DNA marker; Lane 2-11: Different hairy roots plantlets; Lane 12: LBA9402; Lane 13: Untransformed roots; Lane 14: Mili Q water); (F) The hairy root induced by LBA9402; (G) The wild type root. (A)-(D), (F), (G) Bars=20 μm