水稻减数分裂染色体分析方法
程新杰1,于恒秀1,程祝宽2,*(
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Protocols for Analyzing Rice Meiotic Chromosomes
Xinjie Cheng1,Hengxiu Yu1,Zhukuan Cheng2,*()
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图1. 水稻减数分裂粗线期染色体
(A) 水稻花粉母细胞减数分裂粗线期染色体的荧光原位杂交结果, 红色为端粒探针pAtT4的杂交信号, 蓝色为DAPI染色的粗线期染色体(普通荧光显微镜下观察); (B) 水稻花粉母细胞减数分裂粗线期染色体的免疫荧光染色结果, 绿色为PAIR3荧光信号, 红色为ZEP1荧光信号(超分辨荧光显微镜下观察)。Bars=5 µm
Figure 1. Characterization of rice meiotic pachytene chromosomes
(A) Fluorescent in situ hybridization using rice pachytene chromosomes probed with the telomeric sequence pAtT4 (red); Chromosomes stained with DAPI (blue) (Images taken with a regular fluorescence microscope); (B) Immunodetection of PAIR3 (green) and ZEP1 (red) in rice pachytene chromosomes (Images taken with a super resolution microscope). Bars=5 µm
