图1. 牛血清白蛋白(BSA)体外S-亚硝基化修饰的分析检测
使用biotin-maleimide标记BSA样品, 经SDS-PAGE胶分离后进行免疫印迹分析。分别采用anti-biotin和anti-BSA抗体作为第一抗体(primary antibodies; 1:20 000稀释), anti-mouse IgG为第二抗体(secondary antibodies)。样品信号用SuperSignal Western Femto Maximun Sensitivity Substrate Kit检测。曝光时间分别为20秒(上)和30秒(下)。GSH: 谷胱甘肽; GSNO: S-亚硝基谷胱甘肽; Asc: 抗坏血酸盐

Figure 1. In vitro analysis of S-nitrosylated bovine serum albumin (BSA)
BSA is labelled with biotin-maleimide, subjected to SDS- PAGE and western blotting. Anti-biotin and anti-BSA antibodies are used as primary antibodies, respectively (dilution in 1:20 000) and anti-mouse IgG as a secondary antibody. Signals are detected by using the SuperSignal Western Femto Maximun Sensitivity Substrate Kit. The blots are exposed for 20 sec (top) and 30 sec (bottom), respectively. GSH: Glutathione; GSNO: S-nitrosoglutathione; Asc: Sodium ascorbate