甘蔗节间伸长过程赤霉素生物合成关键基因的表达及相关植物激素动态变化

范业赓,丘立杭,黄杏,周慧文,甘崇琨,李杨瑞,杨荣仲,吴建明,陈荣发

Expression Analysis of Key Genes in Gibberellin Biosynthesis and Related Phytohormonal Dynamics During Sugarcane Internode Elongation

Yegeng Fan,Lihang Qiu,Xing Huang,Huiwen Zhou,Chongkun Gan,Yangrui Li,Rongzhong Yang,Jianming Wu,Rongfa Chen

表1 实时荧光定量PCR引物序列

Table 1 Primers for real-time quantitative PCR

Genes Sequences of primers (5'-3') Tm (°C) Product length (bp) 25S rRNA F: CGAATACGAACCGTGAAAGC 58.4 164 R: ACAATGATAGGAAGAGCCGACA 59.0 GA20-Oxidase1 F: CCGTCGTGGACTACTTCG 54.0 205 R: CACGGCGGGTAGTAGTTGAG 58.3 GID1 F: GGTCATCCTCTTCTTCCACGG 60.8 119 R: AGTTGACGGACACCACGACG 61.3 GAI F: GCGACGACAAGGATGAGGA 58.8 186 R: TGAAGCGGTCAAGGAATGTG 59.2