葡萄NCED基因家族进化及表达分析

王小龙,刘凤之,史祥宾,王孝娣,冀晓昊,王志强,王宝亮,郑晓翠,王海波

Evolution and Expression of NCED Family Genes in Vitis vinifera

Xiaolong Wang,Fengzhi Liu,Xiangbin Shi,Xiaodi Wang,Xiaohao Ji,Zhiqiang Wang,Baoliang Wang,Xiaocui Zheng,Haibo Wang

表1 RT-PCR分析所用引物序列

Table 1 The primer sequences used for quantitative RT-PCR

Gene name Forward primer (5'-3') Reverse primer (5'-3') VvNCED1 GCTGGAGAAGCTGATAGTGAAG GAAGATACCCAATGACCGGAAG VvNCED2 GGCACTTTCGGAGGTTGATAA TGGATGAGCAGTGAAGGAATG VvNCED3 CGGTGGAGATGGTGAGAATAGA CACTGCTGCGTACACGTATTT VvNCED4 CTCAGCAGTAGGTGATCCTTTG CAGGCTCGTACATTCTCTTAGC VvNCED6 CTCGTGATTTGGGCTCTTTCT GCTTGATGATGTGTGCTTTGG VvNCED7 CGCTCTTCTTCTTCCTCACTAC GGCGTTCCCTCTTCTACTATTG VvNCED9 CCATGGACTTCCCGATGATAAA ATCCCACAACTAGAGCTTGC VvNCED10 CAGGGAGGTGTTGAAGAAGATG CCCTTTGAGGCAGTGTGATT VvActin TACAATTCCATCATGAAGTGTGATG TTAGAAGCACTTCCTGTGAACAATG