CRISPR/Cas9系统在植物基因组编辑中技术改进与创新的研究进展
苏钺凯,邱镜仁,张晗,宋振巧,王建华(
)
)
Recent Progress in Evolutionary Technology of CRISPR/Cas9 System for Plant Genome Editing
Yuekai Su,Jingren Qiu,Han Zhang,Zhenqiao Song,Jianhua Wang()
)
图1. CRISPR/Cas9基因组编辑原理(
来自酿脓链球菌(Streptococcus pyogenes)的Cas9蛋白(SpCas9)与包含20 nt识别序列的向导RNA (sgRNA)结合, 然后定向切割位于原型间隔序列毗邻基序(PAM)区域上游的靶序列造成双链断裂(DSB), 最终借助生物体内自身修复实现基因组编辑。
Figure 1. The schematic diagram of CRISPR/Cas9 genome editing (
The Cas9 protein from Streptococcus pyogenes (SpCas9) and associated guide RNA (sgRNA), containing a 20 nt recognition sequence, will cleave a target sequence located upstream of the protospacer adjacent motif (PAM) region, resulting in double-strand break (DSB). Ultimately use the repair in the organism to achieve genome editing.
