走马胎的组织培养与快速繁殖

唐凤鸾,赵健,赵志国,夏科,仇硕

Tissue Culture and Rapid Propagation of Ardisia gigantifolia

Fengluan Tang,Jian Zhao,Zhiguo Zhao,Ke Xia,Shuo Qiu

表1 走马胎腋芽增殖的正交设计与结果

Table 1 Design and result of orthogonal test on axillary buds propagation of Ardisia gigantifolia

No. Plant growth regulator (mg·L-1) Average height (cm) Average propagation coefficient State of growth 6-BA ZT NAA 1 0.1 0.1 0 5.5 2.5 Buds grew generally; leaves were medium, dark red 2 0.1 0.2 0.1 8.0 3.5 Buds grew well; leaves were big, dark red 3 0.1 0.4 0.5 8.3 3.5 Buds grew well; leaves were big, dark red 4 0.1 0.8 1.0 6.5 3.0 Buds grew generally; leaves were big, dark red 5 0.5 0.1 0.1 8.6 4.3 Buds grew well; leaves were big, dark red 6 0.5 0.2 0 7.2 3.5 Buds grew well; leaves were medium, dark red 7 0.5 0.4 1.0 7.5 4.0 Buds grew well; leaves were big, dark red 8 0.5 0.8 0.5 6.5 3.2 Buds grew well; leaves were big, dark red 9 1.0 0.1 0.5 5.5 4.0 Buds grew generally; leaves were medium; there are two or four lateral buds 10 1.0 0.2 1.0 6.5 4.5 Buds grew well; leaves were medium; there are two or four lateral buds 11 1.0 0.4 0 4.8 3.5 Buds grew generally; leaves were small; there are one or three lateral buds 12 1.0 0.8 0.1 3.5 3.0 Buds grew generally; leaves were small; there are one or two lateral buds 13 2.0 0.1 1.0 3.0 2.5 Buds grew poorly; leaves were small and yellow; base of seedlings had many calli 14 2.0 0.2 0.5 3.0 1.8 Seedlings had variation; leaves were small and yellow; base of seedlings had many calli 15 2.0 0.4 0.1 2.0 1.5 Buds grew poorly; leaves were very small and some of them had fell off; base of seedlings had many calli 16 2.0 0.8 0 1.8 0.9 Buds grew poorly and died partially