火龙果茎基因组DNA提取方法改良
农全东1,2,3,张明永1,2,张美2,简曙光2,陆宏芳2,夏快飞1,2,*,文和明3,*
Improvement in Extraction Methods of Genomic DNA from Pitaya Stems
Quandong Nong1,2,3,Mingyong Zhang1,2,Mei Zhang2,Shuguang Jian2,Hongfang Lu2,Kuaifei Xia1,2,*,Heming Wen3,*

图2. 火龙果DNA、酶切及ISSR扩增电泳图
(A) 3种改进方法提取的DNA电泳图(M: DNA marker; 1-3: 用“改进一”方法提取的DNA; 4-6: 用“改进二”方法提取的DNA; 7-9: 用“改进三”方法提取的DNA); (B) 用“改进三”方法提取的火龙果DNA及酶切产物电泳图(M: DNA marker; 1-7: DNA; 8-14: HindIII酶切产物; 15-21: HindIII和EcoRI酶切产物; 8-14及15-21所用模板DNA分别对应于1-7泳道中的DNA); (C) ISSR扩增电泳图(M: DNA marker; 1-7: 以“改进三”方法提取的7个DNA为模板进行ISSR扩增的产物)。

Figure 2. Electrophoretograms of pitaya DNA, enzyme digestion and ISSR amplification
(A) Electrophoretogram of DNA extracted by 3 different improved methods (M: DNA marker; Lane 1-3: DNA extracted by improved 1 method; Lane 4-6: DNA extracted by improved 2 method; Lane 7-9: DNA extracted by improved 3 method). (B) Electrophoretogram of pitaya DNA extracted by improved 3 method and enzyme digestion (M: DNA marker; Lane 1-7: DNA; Lane 8-14: DNA digested by HindIII; Lane 15-21: DNA digested by HindIII and EcoRI; The DNA template of 8-14 and 15-21 is corresponding to the DNA in 1-7 lanes, respectively). (C) Electrophoretogram of ISSR amplification (M: DNA marker; Lane 1-7: Products of 7 different DNA templates ISSR amplification).