火龙果茎基因组DNA提取方法改良

农全东,张明永,张美,简曙光,陆宏芳,夏快飞,文和明

Improvement in Extraction Methods of Genomic DNA from Pitaya Stems

Quandong Nong,Mingyong Zhang,Mei Zhang,Shuguang Jian,Hongfang Lu,Kuaifei Xia,Heming Wen

表1 3种改进CTAB+Tris-HCl洗涤法提取的火龙果DNA的OD₂₆₀/OD₂₈₀和OD₂₆₀/OD₂₃₀值

Table 1 The OD₂₆₀/OD₂₈₀ and OD₂₆₀/OD₂₃₀ of DNA extracted from pitaya by 3 different improved CTAB+Tris-HCl washing methods

No.	Improved 1		Improved 2		Improved 3
No.	OD₂₆₀/OD₂₈₀	OD₂₆₀/OD₂₃₀	OD₂₆₀/OD₂₈₀	OD₂₆₀/OD₂₃₀	OD₂₆₀/OD₂₈₀	OD₂₆₀/OD₂₃₀
1	1.99	1.38	3.83	0.48	2.06	1.51
2	1.90	1.16	2.12	0.92	2.04	1.79
3	2.03	1.30	2.69	0.63	2.05	1.65