SmGGPPS2对丹参酮合成的影响
化文平1,2,陈尘3,4,智媛2,刘莉1,王喆之2,李翠芹2,*(
)
)
Effect of SmGGPPS2 Expression on Tanshinones Biosynthesis in Salvia miltiorrhiza
Wenping Hua1,2,Chen Chen3,4,Yuan Zhi2,Li Liu1,Zhezhi Wang2,Cuiqin Li2,*()
)
图1. 丹参转基因株系及其DNA水平的PCR检测
(A) SmGGPPS2过表达代表性株系(OG2-3)、SmGGPPS2 RNA干涉表达代表性株系(IG2-6)及野生型对照(Bar=2 cm); (B) SmGGPPS2过表达转基因株系(1-13); (C) SmGGPPS2- RNAi转基因株系(1-20)。M: DNA标准量DL2000; WT: 野生型; c: 阴性对照; p: 阳性对照
Figure 1. Phenotypes and PCR-screening of the transgenic lines of Salvia miltiorrhiza
(A) SmGGPPS2-overexpression transgenic line (OG2-3), SmGGPPS2-RNAi transgenic line (IG2-6), and wild type (Bar= 2 cm); (B) SmGGPPS2-overexpression transgenic lines (1-13); (B) SmGGPPS2-RNAi transgenic lines (1-20). M: DNA marker DL2000; WT: Wild type; c: Negative control; p: Positive control
