SmGGPPS2对丹参酮合成的影响

化文平,陈尘,智媛,刘莉,王喆之,李翠芹

Effect of SmGGPPS2 Expression on Tanshinones Biosynthesis in Salvia miltiorrhiza

Wenping Hua,Chen Chen,Yuan Zhi,Li Liu,Zhezhi Wang,Cuiqin Li

表2 本研究中qPCR检测所用引物

Table 2 Primers used for qPCR in this study

Primer name Sequence (5′→3′) Primer name Sequence (5′→3′) ActinS AGGAACCACCGATCCAGACA SmDXS1S TGAGAGCGACTACGACTGCTTTGG ActinR GGTGCCCTGAGGTCCTGTT SmDXS1R CCCATCCAGATTGGCAGTAGGC SmHMGR1S GCAACCATCTACTCTCGTCCCA SmDXS2S GGTCGAGGAACTGGAGGGATTG SmHMGR1R GTGCTCCATGAGCTGCATCAG SmDXS2R CGTCAGGATTTCGTGCGGATA SmHMGR2S GGGTTCAACTACGAGGCCATACTG SmDXS3S CACGAATGGGCTGCCAAAAT SmHMGR2R TGTTTGTGCTCGCCACCAGG SmDXS3R CCATCGAATCCAATGAAGCCAC SmHMGR3S AGTCTCGTGATGTCCCTGCTCG SmGGPPS1S GGGGCTATTTTGGGAGGTGGAA SmHMGR3R GCCTCAACCTGCTTGGCGTA SmGGPPS1R CAGCAGCTTGGGATACGTGGTC SmIPI1S AGCGTGCATCCAAATCCAGAC SmGGPPS2S CGGTCTCCTCTCAACCTCTGTCAA SmIPI1R GATAGCTTCAAGCCCCCCTCA SmGGPPS2R CTCCTTCATCTGGACCACTGCCT SmCPS1S ATGGATGGGCAGCAGCAGTAAA SmGGPPS3S GGCCAGTGCTCTGCTGTCTGTG SmCPS1R CGTCCTCAACAACGTCCTGATGTATT SmGGPPS3R TCGGCCACCTCCATCGCTT