SmGGPPS2对丹参酮合成的影响
|
|
化文平,陈尘,智媛,刘莉,王喆之,李翠芹
|
Effect of SmGGPPS2 Expression on Tanshinones Biosynthesis in Salvia miltiorrhiza
|
|
Wenping Hua,Chen Chen,Yuan Zhi,Li Liu,Zhezhi Wang,Cuiqin Li
|
|
表1 本研究中载体构建及检测所用引物
|
Table 1 Primers used for vector construction and detection in this study
|
|
| Primer name | Sequence (5′→3′) | | OG2S (KpnI) | GGGGTACCGCGAAGAGCGTGGAAGC- AGA | | OG2R (BamHI) | CGGGATCC GGCAATAGCAATCAAGGGAGCA | | G2i1S (EcoRI) | CGGAATTC CTCCTCTCAACCTCTGTCAAAACTC | | G2i1R (KpnI) | GGGGTACCTCATCTGGACCACTGCC- TCC | | G2i2S (BamHI) | CGGGATCC CTCCTCTCAACCTCTGTCAAAACTC | | G2i2R (HindIII) | CCCAAGCTTTCATCTGGACCACTGCC- TCC | | 35SF | GCCGTGAAGACTGGCGAACA | | 35SR | AGGAAGGGTCTTGCGAAGGATAGT |
|
|
|