铁棍山药微型块茎遗传转化体系的建立

李俊华^1,^2,^3,^†,刘世宇^1,^†,李成龙¹,韩林林¹,董亚辉¹,张晓丽^1,^2,³,赵喜亭^1,^2,³,李明军^1,^2,^3,^*(

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Establishment of a Genetic Transformation System for Dioscorea opposita Using Microtuber

Junhua Li^1,^2,^3,^†,Shiyu Liu^1,^†,Chenglong Li¹,Linlin Han¹,Yahui Dong¹,Xiaoli Zhang^1,^2,³,Xiting Zhao^1,^2,³,Mingjun Li^1,^2,^3,^*(

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图3. 筛选剂对铁棍山药微型块茎苗再生及再生苗生根的影响
(A)-(F) 不同浓度Hyg对微型块茎苗再生的影响, Hyg浓度分别为0、5、10、15、20和25 mg·L^-1; (G)-(L) 不同浓度Kan对微型块茎苗再生的影响, Kan浓度分别为0、80、100、120、140和160 mg·L^-1; (M) 不同浓度Hyg对再生苗生根的影响; (N) 不同浓度Kan对再生苗生根的影响。(A)-(L) Bars=0.5 cm; (M), (N) Bars=2 cm

Figure 3. Effects of antibiotic on microtuber regeneration and rooting of regenerated seedlings in Dioscorea opposita cv. ‘Tiegun’
(A)-(F) Effects of different concentrations of Hyg on microtuber regeneration, the concentrations of Hyg are 0, 5, 10, 15, 20, 25 mg·L^-1, respectively; (G)-(L) Effects of different concentrations of Kan on microtuber regeneration, the concentrations of Kan are 0, 80, 100, 120, 140, 160 mg·L^-1, respectively; (M) Effects of different concentrations of Hyg on rooting of regenerated plantlets; (N) Effects of different concentrations of Kan on rooting of regenerated plantlets. (A)-(L) Bars=0.5 cm; (M), (N) Bars=2 cm