铁棍山药微型块茎遗传转化体系的建立

李俊华,刘世宇,李成龙,韩林林,董亚辉,张晓丽,赵喜亭,李明军

Establishment of a Genetic Transformation System for Dioscorea opposita Using Microtuber

Junhua Li,Shiyu Liu,Chenglong Li,Linlin Han,Yahui Dong,Xiaoli Zhang,Xiting Zhao,Mingjun Li

表3 不同浓度筛选剂对铁棍山药微型块茎切片再生的影响

Table 3 Effects of antibiotic concentrations on regeneration of microtuber slices in Dioscorea opposita cv. ‘Tiegun’

Concentration of Hyg (mg·L-1) Regeneration (%) Concentration of Kan (mg·L-1) Regeneration (%) Growth of seedlings 0 60.00±2.00 0 65.56±2.52 Strong growth vigor 5 55.56±1.15 80 48.89±1.53 Well 10 33.33±2.00 100 34.44±1.53 Slow, albefaction in some seedlings 15 25.56±0.58 120 8.89±3.06 Slow, albefaction in many seedlings 20 2.22±1.15 140 1.11±0.58 Growth stopped, albefaction seriously 25 0 160 0 All dead