铁棍山药微型块茎遗传转化体系的建立
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李俊华,刘世宇,李成龙,韩林林,董亚辉,张晓丽,赵喜亭,李明军
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Establishment of a Genetic Transformation System for Dioscorea opposita Using Microtuber
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Junhua Li,Shiyu Liu,Chenglong Li,Linlin Han,Yahui Dong,Xiaoli Zhang,Xiting Zhao,Mingjun Li
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表2 引物序列
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Table 2 Sequences of primers
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| Primers | Sequences (5'-3') | | DoSERK2-OE-F | ATGACGGCTTGGGTTTTC | | DoSERK2-OE-R | TCACCTCGGACCAGATAGC | | DoSERK2-RNAi-F | CAGATGATACAGAAAAGCACCG | | DoSERK2-RNAi-R | TAACTTTCGGTAGAGCGGAC | | DoActin-F | CTCATTGATCGGCATGGAAGC | | DoActin-R | GGGGAACATAGTTGAACCACCAC | | DoSERK2-qRT-F | TATCTGGACCAGTTCCATCC | | DoSERK2-qRT-R | CTTCAGCAGGCACATCATAG |
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