铁棍山药微型块茎遗传转化体系的建立

李俊华,刘世宇,李成龙,韩林林,董亚辉,张晓丽,赵喜亭,李明军

Establishment of a Genetic Transformation System for Dioscorea opposita Using Microtuber

Junhua Li,Shiyu Liu,Chenglong Li,Linlin Han,Yahui Dong,Xiaoli Zhang,Xiting Zhao,Mingjun Li

表1 铁棍山药遗传转化和植株再生培养基

Table 1 Medium used for genetic transformation and plant regeneration of Dioscorea opposita cv. ‘Tiegun’

Media Composition MS0 MS major salts, MS minor salts and MS vitamins +30 g·L-1 sucrose+6 g·L-1 fungible agar, pH5.8- 6.2 MS0 1 MS0+1 mg·L-1 6-BA+1 mg?L-1 IAA MS0 2 MS0+2 mg?L-1 PP333+0.05 mg?L-1 NAA MS0 1-A MS0 1+100 μmol?L-1 Acetosyringone (AS) MS0 1-T MS0 1+500 mg?L-1 Timentin (Tim) MS0 1-TH MS0 1+500 mg?L-1 Tim+15 mg?L-1 Hyg MS0 1-TK MS0 1+500 mg?L-1 Tim+120 mg?L-1 Kan MS0 2-H MS0 2+20 mg?L-1 Hyg MS0 2-K MS0 2+160 mg?L-1 Kan