图3. 外源诱导OsSAPP3异源过表达导致转基因拟南芥无法正常萌发
(A)-(C) DEX诱导36小时, 各转基因种子与对照种子萌发情况; (D)-(F) DEX诱导48小时, 各转基因种子与对照种子萌发情况; (G)-(I) DEX诱导72小时, 各转基因种子与对照种子萌发情况; (J) 外源诱导不同时长, OsSAPP3基因的表达变化情况; (K) 外源诱导不同时长, WRKY6基因的表达变化情况; (L) GVG-OsSAPP3转基因拟南芥纯合株系的基因表达水平。数据为平均值±标准差, n=10, 实验经3次生物学重复。* P<0.05, ** P<0.01。Bars=1 cm

Figure 3. Exogenously induced OsSAPP3 heterologous over expression resulted in the inability of germination of transgenic Arabidopsis
(A)-(C) DEX induced 36 h, the germination of transgenic and control seeds; (D)-(F) DEX induced 48 h, the germination of transgenic and control seeds; (G)-(I) DEX induced 72 h, the germination of transgenic and control seeds; (J) The expression of OsSAPP3 induced by exogenous factors at different times; (K) Exogenous induction of WRKY6 expression changes at different times; (L) Gene expression levels of GVG-OsSAPP3 transgenic Arabidopsis homozygous lines. Values are means±SD of one representative biological replicate (n=10) out of three. * P<0.05, ** P<0.01. Bars=1 cm