利用荧光标记SSR鉴别21个茶花新品种
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陶乃奇,张斌,刘信凯,周和达,钟乃盛,严丹峰,张敏,高继银,张文驹
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Identification of 21 New Camellia Hybrid Varieties by Fluorescence-labelled Simple Sequence Repeat Markers
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Naiqi Tao,Bin Zhang,Xinkai Liu,Heda Zhou,Naisheng Zhong,Danfeng Yan,Min Zhang,Jiyin Gao,Wenju Zhang
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表2 12对SSR引物信息
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Table 2 Information of 12 primer pairs in this study
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| No. | Primer sequences (5′-3′) | Repeat motifs | Ta (°C) | References | | 478 | F: CAACACCACCAACAAGA | (AAAGG)4 | 53 | Liu et al., 2008 | | R: GATATGAGATCCGTCCC | | SSR2 | F: TATTGCCTACGACCATTTCCA | (GA)14 | 56 | Kaundun and Matsumoto, 2002 | | R: TTTGAGTTCGTTGCCTTCTCT | | CamsinM11 | F: GCATCATTCCACCACTCACC | (CA)12 | 60 | Freeman et al., 2004 | | R: GTCATCAAACCAGTGGCTCA | | CamSSR01 | F: CCAACAAGAATCAGGAAGAG | (AAT)6 | 54 | In this study | | R: ATCCAACGGTGGTAGACGAG | | CamSSR02 | F: AGTTCCGCCTCCAGTTTGAC | (ACG)7 | 54 | In this study | | R: GGACCGAGAGGTAACAGTGG | | CamSSR03 | F: GCCACTACCCTCTTTACACC | (CAC)7 | 55 | In this study | | R: TTCTCTTCCTCTTTCTTCCC | | CamSSR04 | F: ATGTGTTGAGTAGCGAGCGT | (AT)10 | 56 | In this study | | R: TTGTCCATCTTTATGTAGGG | | CamSSR05 | F: GCAAACACCAACTGATTACC | (TA)10 | 56 | In this study | | R: TTCCATACAACTCAACCAAA | | CamSSR06 | F: GGTTTGGAAAAAGGACACGC | (GCC)7 | 58 | In this study | | R: AATCTGCCTCTGGTAGTCCG | | CamSSR07 | F: TCTCATCCCCATCTTTATCC | (TCC)7 | 58 | In this study | | R: GTTCCCTGCTGCTGTTGTTA | | CamSSR08 | F: TCACCAGTCACTTTCCCTCC | (AC)10 | 58 | In this study | | R: CCACCAAAAGGCACAATACC | | CamSSR09 | F: CATCATCCATCAAACCGTCC | (AT)10 | 58 | In this study | | R: GAAGGCACATTGGTTCTGGG |
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