异源过表达<i>OsATG8b</i>基因提高转基因拟南芥的氮/碳胁迫耐受性和产量

异源过表达OsATG8b基因提高转基因拟南芥的氮/碳胁迫耐受性和产量

甄晓溪,刘浩然,李鑫,徐凡(

),张文忠(

)

Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis

Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu(

),Wenzhong Zhang(

)

图6. 过表达OsATG8b转基因拟南芥中氮代谢关键基因的表达变化
将7天苗龄的35S-OsATG8b转基因和野生型(WT)拟南芥移至全氮(NS)和缺氮(ND) 1/2MS培养基处理14天。(A) 35S-OsATG8b转基因和WT拟南芥中地上部氮代谢相关基因相对表达水平; (B) 地下部氮代谢相关基因相对表达水平。数据为平均值±标准差, n=10, *和**分别表示各转基因株系与WT之间差异显著(P<0.05)和极显著(P<0.01) (Student’s t-test), 实验经3次生物学重复。

Figure 6. Overexpression of OsATG8b in Arabidopsis changes the expression of genes in nitrogen metabolic
7-day-old seedlings of transgenic lines and wild type (WT) were transferred to 1/2MS medium with sufficient (NS) or deficient (ND) nitrogen for 14 days. (A) The expression of genes related to nitrogen metabolic in rosette leaves of 35S-OsATG8b transgenic lines and WT; (B) The expression of genes related to nitrogen metabolic in roots of 35S-OsATG8b transgenic lines and WT. Values are means±SD, n=10, * and ** indicate significant (P<0.05) and extremely significant (P<0.01) differences between transgenic lines and WT (Student’s t-test), respectively, three biological replicates were performed.