异源过表达OsATG8b基因提高转基因拟南芥的 氮/碳胁迫耐受性和产量
甄晓溪,刘浩然,李鑫,徐凡(
),张文忠()
),张文忠()
Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis
Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu(),Wenzhong Zhang()
),Wenzhong Zhang()
图5. 缺氮条件下过表达OsATG8b转基因拟南芥中的自噬活性增加
将苗龄7天的35S-OsATG8b转基因(L-14)和野生型(WT)拟南芥幼苗转移至含有1 μmol·L-1 ConA的缺氮培养液中处理12小时后进行MDC染色, 于激光共聚焦显微镜下观察自噬体荧光。Bars=10 μm
Figure 5. Overexpression of OsATG8b in Arabidopsis enhanced the autophagic activity under N deficient condition
7-day-old seedlings of transgenic line (L-14) and wild type (WT) were transferred to in N-deficient (ND) liquid medium with 1 μmol·L-1 ConA for 12 h, MDC-stained autophagosomes in leaves were observed by confocal microscopy. Bars=10 μm
