异源过表达OsATG8b基因提高转基因拟南芥的 氮/碳胁迫耐受性和产量
甄晓溪,刘浩然,李鑫,徐凡(
),张文忠()
),张文忠()
Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis
Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu(),Wenzhong Zhang()
),Wenzhong Zhang()
图4. 过表达OsATG8b促进转基因拟南芥抵抗黑暗诱导的碳胁迫
(A) 对苗龄7天的35S-OsATG8b转基因拟南芥和野生型(WT)黑暗处理10天后记录表型(左为处理前, 右为处理后); (B) 叶绿素含量测定。数据为平均值±标准差, n=10, *表示各转基因株系与WT之间差异显著(P<0.05) (Student’s t-test), 实验经3次生物学重复。Bars=5 mm
Figure 4. Overexpression of OsATG8b in Arabidopsis enhanced tolerance to carbon starvation induced by dark treatment
(A) 7-day-old seedlings of 35S-OsATG8b transgenic lines and wild type (WT) were transferred to darkness for 10 days (The left is before treatment, and the right is after treatment); (B) The chlorophyll content determination. Values are means±SD, n=10, * indicate significant difference between transgenic lines and WT (P<0.05) (Student’s t-test), three biological replicates were performed. Bars=5 mm
