图3. 过表达OsATG8b促进转基因拟南芥抵抗缺氮胁迫
(A) 将苗龄7天的35S-OsATG8b转基因和野生型(WT)拟南芥幼苗分别移入全氮(NS)和缺氮(ND)1/2MS培养基中培养9天后的表型; (B)-(D) 分别为全氮(NS)和缺氮(ND)处理9天的35S-OsATG8b转基因和WT拟南芥幼苗的鲜重, 叶绿素和可溶性蛋白含量; (E) 苗龄7天的35S-OsATG8b转基因和WT拟南芥幼苗全氮(NS)和缺氮(ND)垂直培养9天后的表型; (F)-(H) 分别为全氮(NS)和缺氮(ND)处理9天的35S-OsATG8b转基因和WT拟南芥幼苗的主根长、地上部和地下部鲜重; (I), (J) 分别为苗龄10天的Pro_OsATG8b-GUS转基因拟南芥经缺氮处理和10 μmol·L^-1水杨酸(SA)处理24小时后的GUS组织化学染色结果。Mock代表未经处理的拟南芥。数据为平均值±标准差, n=16, *和**分别表示各转基因株系与WT之间差异显著(P<0.05)和极显著(P<0.01) (Student’s t-test), 实验经3次生物学重复。Bars=5 mm

Figure 3. Overexpression of OsATG8b enhances tolerance to N deficiency in transgenic Arabidopsis
(A) 7-day-old seedlings of 35S-OsATG8b transgenic lines and wild type (WT) were transferred to 1/2MS medium for horizontal culture with sufficient (NS) or deficient (ND) N for 9 days. (B)-(D) The fresh weight, chlorophyll content and soluble protein content in rosette leaves of WT and 35S-OsATG8b transgenic Arabidopsis under NS or ND for 9 days, respectively; (E) The phenotype of 7-day-old seedlings of 35S-OsATG8b transgenic lines and WT were transferred to vertical plates with NS or ND for 9 days; (F)-(H) The primary root length, the shoot weight and the root weight of WT and transgenic Arabidopsis lines under NS or ND for 9 days, respectively; (I), (J) 10-day-old seedlings of Pro_OsATG8b-GUS transgenic Arabidopsis were transferred to ND and 10 μmol·L^-1 SA for 24 h, respectively. Mock represented that the seedlings without treated. Values are means±SD, n=16, * and ** indicate significant (P<0.05) and extremely significant (P<0.01) differences between transgenic lines and WT (Student’s t-test), three biological replicates were performed. Bars=5 mm