异源过表达OsATG8b基因提高转基因拟南芥的 氮/碳胁迫耐受性和产量
甄晓溪,刘浩然,李鑫,徐凡(),张文忠()
Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis
Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu(),Wenzhong Zhang()

图2. 过表达OsATG8b促进转基因拟南芥生长发育
将苗龄8天的野生型(WT)和35S-OsATG8b转基因拟南芥幼苗移入蛭石-营养土(1:3, v/v)中。(A) 苗龄14天的35S-OsATG8b转基因拟南芥和WT幼苗中OsATG8b的表达水平; (B) 由上至下依次为移苗后10、17和24天的拟南芥表型; (C) 移苗后42天的表型; (D) 不同苗龄的35S-OsATG8b转基因拟南芥和WT的最大莲座叶半径; (E) 株高; (F) 总叶绿素含量; (G) 可溶性蛋白含量。Days: 萌发后天数。图中数据为平均值±标准差, n=24, * P<0.05, ** P<0.01 (Student’s t-test), 实验经3次生物学重复。Bars=1 cm

Figure 2. Over-expression of OsATG8b promotes growth and development of transgenic Arabidopsis
8-day-old seedlings were transferred to vermiculite-nutritional soil (1:3, v/v). (A) Expression level of OsATG8b in 14-day-old seedlings of 35S-OsATG8b transgenic lines and wild type (WT); (B) Panels from top to bottom show phenotypic observations of transgenic lines and WT of Arabidopsis at 10, 17 and 24 days after transfer to soil, respectively; (C) Phenotype of transgenic lines and WT at 42 days after transfer to soil; (D) The maximum rosette radius of 35S-OsATG8b transgenic lines and WT at different seedling age; (E) The plant height; (F) The total chlorophyll content; (G) The soluble protein content. Days: Days after germination. Values are means±SD, n=24, * P<0.05, ** P<0.01 (Student’s t-test), three biological replicates were performed. Bars=1 cm