异源过表达OsATG8b基因提高转基因拟南芥的 氮/碳胁迫耐受性和产量
甄晓溪,刘浩然,李鑫,徐凡(),张文忠()
Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis
Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu(),Wenzhong Zhang()

图1. 氮胁迫处理诱导水稻幼苗叶片及根中OsATG8b基因的表达
(A) 全氮条件下生长14天的水稻幼苗转移至全氮(NS)、低氮(NL)和缺氮(ND)条件下生长1和3天时叶片中OsATG8b基因的表达量; (B) 全氮、低氮和缺氮条件下生长1和3天的水稻根系中OsATG8b基因的表达量。图中数据为平均值±标准差, n=16, 实验经3次生物学重复。**表示全氮条件与低氮和缺氮条件相比差异显著(P<0.01) (Student’s t-test)。

Figure 1. Identification of OsATG8b as a nitrogen deficiency inducible/responsive gene in leaves and roots of rice seedlings
(A) The rice seedlings cultured with N-sufficient (NS) solution for 14 days and transferred to the same NS solution, low N (NL) solution and the N-deficient (ND) solution, the expression of OsATG8b gene in leaves after 1 day and 3 days treatment; (B) The expression of OsATG8b gene in roots after 1 day and 3 days treatment. Values are means±SD, n=16, three biological replicates were performed. ** indicate significant differences in NS solution compared with NL and ND solution (P<0.01) (Student’s t-test).