异源过表达OsATG8b基因提高转基因拟南芥的 氮/碳胁迫耐受性和产量

甄晓溪,刘浩然,李鑫,徐凡,张文忠

Heterologous Overexpression of Autophagy-related Gene OsATG8b from Rice Confers Tolerance to Nitrogen/Carbon Starvation and Increases Yield in Arabidopsis

Xiaoxi Zhen,Haoran Liu,Xin Li,Fan Xu,Wenzhong Zhang

表1 引物信息

Table 1 The information of primers

Primer name Sequence (5′-3′) Function cOsATG8b-F CCATTCAAGTGGATGGCCAAGAGCTCGTTCAAGC Gene cloning cOsATG8b-R GGTGACCTAGAGCAGCCCAAAGGTGTTCTCG Gene cloning cpOsATG8b-F AAGCTTAAAATTAAATAAGACGAACAGTCAAACG Gene cloning cpOsATG8b-R CCATGGCGCTCCTTCCTGCACACAAT Gene cloning rtOsATG8b-F GCTGATCTTACCGTTGGGCA Real-time RT-PCR rtOsATG8b-R ATCAGAGCAGCTGTTGGTGG Real-time RT-PCR rtAtAMT1-F GCCTCTGCTGACTACTCCAACTT Real-time RT-PCR rtAtAMT1-R GACCAGAACCAGTGAGAGACGA Real-time RT-PCR rtAtNR1-F AGGATGGGCTAGTAAGCATAAGG Real-time RT-PCR rtAtNR1-R GCAAACTGAATCATAGGCGGTG Real-time RT-PCR rtAtGS2-F CACCAAACCTTACTCTCTGACA Real-time RT-PCR rtAtGS2-R CACTATCTTCACCAGGTGCTTG Real-time RT-PCR rtAtGDH1-F GCTTTAGCAGCAACAAACAGAA Real-time RT-PCR rtAtGDH1-R TGAGCCAATGCGTTCACTTC Real-time RT-PCR rtACTIN1-F ACCATTGGTGCTGAGCGTTT Real-time RT-PCR rtACTIN1-R CGCAGCTTCCATTCCTATGAA Real-time RT-PCR rtTIP41-F GTATGAAGATGAACTGGCTGACAAT Real-time RT-PCR rtTIP41-R ATCAACTCTCAGCCAAAATCGCAAG Real-time RT-PCR