%A Xiaomin Wang, Honglei Li, Lin Wang, Pengze Zhou, Shengyi Bai, Guohua Li, Fushun Zheng, Xiaorong Tao, Guoxin Cheng, Yanming Gao, Jianshe Li %T Molecular Identification of Tomato Spotted Wilt Virus on Tomato in Yinchuan %0 Journal Article %D 2021 %J Chinese Bulletin of Botany %R 10.11983/CBB21113 %P 715-721 %V 56 %N 6 %U {https://www.chinbullbotany.com/CN/abstract/article_81991.shtml} %8 2021-11-01 %X

In order to determine whether Yinchuan tomato (Lycopersicon esculentum) was damaged by tomato spotted wilt virus (TSWV), 14 disease leaf samples of suspected infected TSWV collected from Yinchuan tomato were identified by the national standard TSWV RT-PCR technique. The cloned N gene sequences were analyzed by multi sequence alignment and phylogenetic tree analysis. Eight of the 14 disease leaf samples amplified the TSWV N gene sequence with a length of 394 bp, and the 8 sequences were completely consistent. The TSWV sequence was relatively close to those from Yunnan tomato, Chinese lettuce (Lactuca sativa), Chinese Iris (Iris tectorum) and Chongqing pepper (Capsicum annuum), while relatively distant to those from Shandong, Heilongjiang, Beijing and the abroad. Detection of TSWV in the 8 PCR positive samples by use of Western bolt showed that TSWV infection existed in these samples. This study proved that there was TSWV infection in Yinchuan tomato. Therefore, it is necessary to speed up the breeding of TSWV resistant tomato varieties.