A New Cereal Seed Treatment Method for Displaying Endosperm Cell Structures Under Micro CT Scanning
- 1Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China
2China National Botanical Garden, Beijing 100093, China
3Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
4Guizhou Institute of Subtropical Crops, Guizhou Academy of Agricultural Sciences, Xingyi 562400, China
Received date: 2024-02-18
Accepted date: 2024-03-30
Online published: 2024-04-02
Abstract
Cereal starch endosperm is the main source of human staple food, but the methods for observing its mature cell structure are not well developed. Micro CT technology, a non-destructive three-dimensional imaging technique, is a powerful tool for studying plant morphology. However, due to the uniform density of cereal starch endosperm, whose cell structures cannot be distinguished clearly by conventional micro CT techniques. In this study, we used phosphotungstic acid to treat ten different kinds of cereal seeds of seven crops, and then dried them by CO2 critical point dryer. We found that the cell structure of the treated endosperms can be clearly displayed by micro CT. Our method provides a new way for studying the structures and functions of cereal starch endosperm cells.
Key words： micro CT; cereal starch endosperm; phosphotungstic acid
Cite this article
Xiuping Xu , Xiaoyu Yang , Min Feng . A New Cereal Seed Treatment Method for Displaying Endosperm Cell Structures Under Micro CT Scanning[J]. Chinese Bulletin of Botany, 2025 , 60(1) : 81 -89 . DOI: 10.11983/CBB24022
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