Establishment of In Vitro Regeneration System of Citrus australasica
Received date: 2023-05-12
Accepted date: 2023-09-19
Online published: 2023-10-10
In order to establish the regeneration system of Citrus australasica, the effects of different plant growth regulators combinations, medium types and dark culture time on callus induction and plant regeneration of C. australasica were studied using stem segments as explants. The results showed that the best medium for adventitious bud induction was 1/2MS+4.0 mg∙L-1 ZT+30.0 g∙L-1 sucrose. Dark culture for 14 days and then light culture had the best promoting effect. The induction rate of callus and adventitious bud was 100%, and the average number of adventitious bud regeneration per explants was 4.83. The optimal rooting medium was 1/2MS+0.5 mg∙L-1 NAA, and the regenerated plants with the 94.43% rooting rate were obtained, and the average number of roots was 3.9. In the mixture of grass carbon:perlite: vermiculite=2:1:1 (v/v/v), tissue culture seedlings had the best growth, and the survival rate was more than 90%. This study established in vitro regeneration system of C. australasica, which laid the foundation for the genetic improvement and rapid propagation of C. australasica fine varieties.
Key words： Citrus australasica; stem segment; tissue culture; in vitro regeneration
Chungang Xie, Zhe Liu, Shusheng Zhang, Haitao Hu . Establishment of In Vitro Regeneration System of Citrus australasica[J]. Chinese Bulletin of Botany, 2023 , 58(6) : 926 -934 . DOI: 10.11983/CBB23060
| [1] | 常贝贝, 屈宜宝, 王智宇, 程晓帆, 杜晓云, 于晓丽, 赵玲玲, 张硕 (2023). 2个苹果新品种高效再生体系建立. 分子植物育种. https://kns.cnki.net/kcms/detail/46.1068.S.20220722.1647.008.html. |
| [2] | 范永梅, 甘霖, 邓秀新 (2003). 冰糖橙胚性愈伤组织的诱导与植株再生. 华中农业大学学报 22, 399-402. |
| [3] | 林颖, 龙自立, 张璐, 叶庆富, 刘永立 (2012). 猕猴桃胚乳再生植株体系的优化. 核农学报 26, 257-261, 310. |
| [4] | 逯锦春, 曹丽娜, 佟冠杰, 王鑫颖, 张利英, 喻锌, 李荟芳, 李彦慧 (2022). 大花银莲花愈伤组织诱导及再生体系的建立. 植物学报 57, 217-226. |
| [5] | 任露露, 张有泽, 黄克林, 宛晓春, 张照亮, 朱木兰, 韦朝领 (2023). 茶树茎段不定芽高效发生体系的建立. 植物学报 58, 308-315. |
| [6] | 魏佳, 路天宇, 王朝胜, 张红叶, 张晶, 王顺利, 李润芝 (2022). 不同粒级园艺基质原料物理性质差异分析. 北京农学院学报 37(4), 13-18. |
| [7] | 杨圣涛, 吕岩, 贺元源, 刘婷婷, 马晓祯 (2021). 基于CT扫描的草炭土孔隙结构分析及渗流模拟. 工程地质学报 29, 1354-1365. |
| [8] | 张郎郎, 张洁, 吕虹霖, 谭彬, 王伟, 程钧, 冯建灿 (2022). 欧洲李叶片再生体系的建立. 果树学报 39, 1945-1953. |
| [9] | 张秀英, 鲁兴凯, 程安富, 胡志芳, 马勉娣, 张丹, 黄国嫣, 陈晨, 全勇, 汪琼 (2022). 基质对苹果砧木M26脱毒组培苗移栽成活率和生长的影响. 中国南方果树 51(5), 150-153. |
| [10] | 张旭红, 王頔, 梁振旭, 孙美玉, 张金政, 石雷 (2018). 欧洲百合愈伤组织诱导及植株再生体系的建立. 植物学报 53, 840-847. |
| [11] | Bernula D, Benk? P, Kaszler N, Domonkos I, Valkai I, Sz?ll?si R, Ferenc G, Ayaydin F, Fehér A, Gémes K (2020). Timely removal of exogenous cytokinin and the prevention of auxin transport from the shoot to the root affect the regeneration potential of Arabidopsis roots. Plant Cell Tissue Organ Cult 140, 327-339. |
| [12] | Cioni E, Migone C, Ascrizzi R, Muscatello B, De Leo M, Piras AM, Zambito Y, Flamini G, Pistelli L (2022). Comparing metabolomic and essential oil fingerprints of Citrus australasica F. Muell (Finger Lime) varieties and their in vitro antioxidant activity. Antioxidants (Basel) 11, 2047. |
| [13] | Conti G, Xoconostle-Cázares B, Marcelino-Pérez G, Hopp HE, Reyes CA (2021). Citrus genetic transformation: an overview of the current strategies and insights on the new emerging technologies. Front Plant Sci 12, 768197. |
| [14] | Dai WH, Castillo C (2007). Factors affecting plant regeneration from leaf tissues of buddleia species. HortScience 42, 1670-1673. |
| [15] | Huang T, Peng SL, Dong GF, Zhang LY, Li GG (2002). Plant regeneration from leaf-derived callus in Citrus grandis (pummelo): effects of auxins in callus induction medium. Plant Cell Tissue Organ Cult 69, 141-146. |
| [16] | Jardak R, Boubakri H, Zemni H, Gandoura S, Mejri S, Mliki A, Ghorbel A (2020). Establishment of an in vitro regeneration system and genetic transformation of the Tunisian ‘Maltese half-blood’ (Citrus sinensis): an agro- economically important variety. 3 Biotech 10, 99. |
| [17] | Long Y, Yang Y, Pan GT, Shen YO (2022). New insights into tissue culture plant-regeneration mechanisms. Front Plant Sci 13, 926752. |
| [18] | Loyola-Vargas VM, Ochoa-Alejo N (2018). An introduction to plant tissue culture:advances and perspectives. In: Loyola-Vargas VM, Ochoa-Alejo N, eds. Plant Cell Culture Protocols. New York: Humana Press. pp. 3-13. |
| [19] | Pe?a L, Pérez RM, Cervera M, Juárez JA, Navarro L (2004). Early events in Agrobacterium-mediated genetic transformation of citrus explants. Ann Bot 94, 67-74. |
| [20] | Poles L, Licciardello C, Distefano G, Nicolosi E, Gentile A, La Malfa S (2020). Recent advances of in vitro culture for the application of new breeding techniques in citrus. Plants (Basel) 9, 938. |
| [21] | Raspor M, Motyka V, Kaleri AR, Ninkovi? S, Tubi? L, Cingel A, ?osi? T (2021). Integrating the roles for cytokinin and auxin in de novo shoot organogenesis: from hormone uptake to signaling outputs. Int J Mol Sci 22, 8554. |
| [22] | Skoog F, Miller CO (1957). Chemical regulation of growth and organ formation in plant tissues cultured in vitro. Symp Soc Exp Biol 11, 118-130. |
| [23] | Zenser N, Ellsmore A, Leasure C, Callis J (2001). Auxin modulates the degradation rate of Aux/IAA proteins. Proc Natl Acad Sci USA 98, 11795-11800. |
/
| 〈 |
|
〉 |
Home