Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’
Received date: 2021-10-22
Accepted date: 2022-04-20
Online published: 2022-04-21
Using the scape as explants, we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv. ‘Fenmeiren’. Our results showed that the survival rate of explant was 95%, which explants were obtained in June and sterilized in the 15% (v/v) sodium hypochlorite solution for 8 minutes. The best proliferation medium was MS + 1.0 mg·L-1 6-BA + 0.004 mg·L-1 TDZ + 0.1 mg·L-1 NAA. After 30 days of cultivation, the monthly proliferation coefficient reached 2.9. On the medium of MS + 0.1 mg·L-1 6-BA + 0.1 mg·L-1 IBA, the tissue culture seedlings did not differentiate and grew vigorously. The optimal rooting medium was 1/2MS + 0.4 mg·L-1 IBA + 20 g·L-1 sucrose, and the rooting rate was 95%. by use of perlite:peat=1:2 (v/v) as the transplanting matrix, the survival rate of transplanting was 85% and the qualified rate was 75%. At present, the large-scale production of this cultivar has been achieved and 2.0 × 105 tissue culture seedlings were produced, which all performed well in the field.
Lü Xiuli, Yu Zequn, Chen Xiangbo, Fu Renjie, Miao Shanshan, Du An . Rapid Propagation Technology and Field Production of Hemerocallis fulva cv. ‘Fenmeiren’[J]. Chinese Bulletin of Botany, 2022 , 57(3) : 350 -357 . DOI: 10.11983/CBB21182
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