Using the scape as explants, we successfully established the tissue culture and rapid propagation technology for the large-scale production of Hemerocallis fulva cv. ‘Fenmeiren’. Our results showed that the survival rate of explant was 95%, which explants were obtained in June and sterilized in the 15% (v/v) sodium hypochlorite solution for 8 minutes. The best proliferation medium was MS + 1.0 mg·L^-1 6-BA + 0.004 mg·L^-1 TDZ + 0.1 mg·L^-1 NAA. After 30 days of cultivation, the monthly proliferation coefficient reached 2.9. On the medium of MS + 0.1 mg·L^-1 6-BA + 0.1 mg·L^-1 IBA, the tissue culture seedlings did not differentiate and grew vigorously. The optimal rooting medium was 1/2MS + 0.4 mg·L^-1 IBA + 20 g·L^-1 sucrose, and the rooting rate was 95%. by use of perlite:peat=1:2 (v/v) as the transplanting matrix, the survival rate of transplanting was 85% and the qualified rate was 75%. At present, the large-scale production of this cultivar has been achieved and 2.0 × 10⁵ tissue culture seedlings were produced, which all performed well in the field.