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Chinese Bulletin of Botany >

2014 , Vol. 49 >Issue 1: 120 - 126

DOI: https://doi.org/10.3724/SP.J.1259.2014.00120

A Method to Improve Sample Preparation for Observing Stomata Guard Cells in Triticum aestivum

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  • Institute of Crop Science, Chinese Academy of Agricultural Science/National Center of Space Mutagenesis for Crop Improvement, Beijing 100081, China

Received date: 2013-03-11

  Revised date: 2013-07-23

  Online published: 2014-01-01

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Abstract

We developed an efficient whole-leaf clearing method for observing stomata guard cells of anther-derived plants in Triticum aestivum. We compared the effect of 4 different clearing agents. With this method, we could obtain a sample for observing stomata guard cells in wheat without mesophyll tearing and shaving. After leaf treatment with glycerite, saturation with chloral hydrate and mixed solution of chloral hydrate and glycerin revealed clear images of stomata guard cells by microscopy.

Cite this article

Linshu Zhao, Luxiang Liu, Jiayu Gu, Huijun Guo, Junhui Li, Yongdun Xie, Shirong Zhao . A Method to Improve Sample Preparation for Observing Stomata Guard Cells in Triticum aestivum[J]. Chinese Bulletin of Botany, 2014 , 49(1) : 120 -126 . DOI: 10.3724/SP.J.1259.2014.00120

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