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Chinese Bulletin of Botany >

2021 , Vol. 56 >Issue 1: 25 - 32

DOI: https://doi.org/10.11983/CBB20148

EXPERIMENTAL COMMUNICATIONS

QTL Mapping of Candidate Genes Involved in Cd Accumulation in Rice Grain

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  • 1College of Chemistry and Life Sciences, Zhejiang Normal University, Jinhua 321004, China
    2State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, China
    3Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Received date: 2020-08-26

  Accepted date: 2020-11-11

  Online published: 2020-11-11

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Abstract

Rice is one of the world’s most important crops, but cadmium (Cd) pollution and Cd accumulation in rice fields pose a serious threat to its yield and quality as well as to human health. How to reduce Cd accumulation in rice has become a major concern. In this study, a japonica rice variety Nekken2 was used as the maternal parent, and an indica rice variety HZ was used as the paternal parent. After F1 generation was obtained by cross-breeding, successive selfings were performed for 120 recombinant inbred lines (RILs) population as the QTL mapping populations. We measured the Cd content in rice grains of each line at the maturity stage. Meanwhile, QTL mapping was performed using genetic map. As a result, a total of 7 QTLs were detected, which were located on chromosome 2, 3, 9, and 12 of rice, with the highest LOD score of 4.97. The qRT-PCR analysis on the candidate genes involved in metal ion stress resistance in these QTL regions showed that the expression level of LOC_Os02g50240, LOC_Os02g52780, LOC_Os09g31200, LOC_Os09g35030 and LOC_Os09g37949 was significantly different from the parents. Combined with the data of concentration of different metal ions in the parents, we speculated that the high expression of LOC_Os02g50240, LOC_Os09g31200 and LOC_Os09g35030 might greatly improve the absorption and tolerance of rice to Cd ion stress. And these genes may be related to the accumulation of Cd in rice grains, and may affect the ability of rice to tolerate Cd stress, which could help for further breeding and screening for rice varieties to tolerate Cd stress, and exploring the molecular regulation mechanism of Cd accumulation in rice.

Key words： rice; cadmium accumulation; QTL mapping; candidate genes; ion stress; genetic map

Cite this article

Chenyang Pan, Hanfei Ye, Weiyong Zhou, Sheng Wang, Mengjia Li, Mei Lu, Sanfeng Li, Xudong Zhu, Yuexing Wang, Yuchun Rao, Gaoxing Dai . QTL Mapping of Candidate Genes Involved in Cd Accumulation in Rice Grain[J]. Chinese Bulletin of Botany, 2021 , 56(1) : 25 -32 . DOI: 10.11983/CBB20148

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References

[1] 黄新元, 赵方杰 (2018). 植物防御素调控水稻镉积累的新机制. 植物学报 53, 451-455.
[2] 王兰, 黄李超, 代丽萍, 杨窑龙, 徐杰, 冷语佳, 张光恒, 胡江, 朱丽, 高振宇, 董国军, 郭龙彪, 钱前, 曾大力 (2014). 利用日本晴/9311重组自交系群体定位水稻成熟期叶形相关性状QTL. 中国水稻科学 28, 589-597.
[3] Abe T, Nonoue Y, Ono N, Omoteno M, Kuramata M, Fukuoka S, Yamamoto T, Yano M, Ishikawa S (2013). Detection of QTLs to reduce cadmium content in rice grains using LAC23/Koshihikari chromosome segment substitution lines. Breed Sci 63, 284-291.
[4] Arisawa K, Uemura H, Hiyoshi M, Takeda H, Saito H, Soda M (2007). Cadmium-induced renal dysfunction and mortality in two cohorts: disappearance of the association in a generation born later. Toxicol Lett 169, 214-221.
[5] Cheng YW, Qi YC, Zhu Q, Chen X, Wang N, Zhao X, Chen HY, Cui XJ, Xu LL, Zhang W (2009). New changes in the plasma-membrane-associated proteome of rice roots under salt stress. Proteomics 9, 3100-3114.
[6] Dubouzet JG, Sakuma Y, Ito Y, Kasuga M, Dubouzet EG, Miura S, Seki M, Shinozaki K, Yamaguchi-Shinozaki K (2003). OsDREB genes in rice, Oryza sativa L., encode transcription activators that function in drought-, high-salt- and cold-responsive gene expression. Plant J 33, 751-763.
[7] Florijn PJ, van Beusichem ML (1993). Uptake and distribution of cadmium in maize inbred lines. Plant Soil 150, 25-32.
[8] Fujimaki S, Suzui N, Ishioka NS, Kawachi N, Ito S, Chino M, Nakamura S (2010). Tracing cadmium from culture to spikelet: noninvasive imaging and quantitative characterization of absorption, transport, and accumulation of cadmium in an intact rice plant. Plant Physiol 152, 1796-1806.
[9] Horiguchi H, Teranishi H, Niiya K, Aoshima K, Katoh K, Sakuragawa N, Kasuya M (1994). Hypoproduction of erythropoietin contributes to anemia in chronic cadmium intoxication: clinical study on Itai-itai disease in Japan. Arch Toxicol 68, 632-636.
[10] Hu DW, Sheng ZH, Li QL, Chen W, Wei XJ, Xie LH, Jiao GA, Shao GN, Wang JL, Tang SQ, Hu PS (2018). Identification of QTLs associated with cadmium concentration in rice grains. J Integr Agric 17, 1563-1573.
[11] Ishikawa S, Abe T, Kuramata M, Yamaguchi M, Ando T, Yamamoto T, Yano M (2010). A major quantitative trait locus for increasing cadmium-specific concentration in rice grain is located on the short arm of chromosome 7. J Exp Bot 61, 923-934.
[12] Liu WQ, Pan XW, Li YC, Duan YH, Min J, Liu SX, Liu LC, Sheng XN, Li XX (2019). Identification of QTLs and validation of qCd-2 associated with grain cadmium concentrations in rice. Rice Sci 26, 42-49.
[13] Livak KJ, Schmittgen TD (2001). Analysis of relative gene expression data using real-time quantitative PCR and the 2?ΔΔCT method. Methods 25, 402-408.
[14] Mao CP, Song YX, Chen LX, Ji JF, Li JZ, Yuan XY, Yang ZF, Ayoko GA, Frost RL, Theiss F (2019). Human health risks of heavy metals in paddy rice based on transfer characteristics of heavy metals from soil to rice. Catena 175, 339-348.
[15] McCouch SR, Cho YG, Yano M, Paul E, Blinstrub M, Morishima H, Kinoshita T (1997). Report on QTL nomenclature. Rice Genet Newsl 14, 11-13.
[16] Miyadate H, Adachi S, Hiraizumi A, Tezuka K, Nakazawa N, Kawamoto T, Katou K, Kodama I, Sakurai K, Takahashi H, Satoh-Nagasawa N, Watanabe A, Fujimura T, Akagi H (2011). OsHMA3, a P1B-type of ATPase affects root-to-shoot cadmium translocation in rice by mediating efflux into vacuoles. New Phytol 189, 190-199.
[17] Mukhopadhyay A, Vij S, Tyagi AK (2004). Overexpression of a zinc-finger protein gene from rice confers tolerance to cold, dehydration, and salt stress in transgenic tobacco. Proc Natl Acad Sci USA 101, 6309-6314.
[18] Nakanishi H, Ogawa I, Ishimaru Y, Mori S, Nishizawa NK (2006). Iron deficiency enhances cadmium uptake and translocation mediated by the Fe2+ transporters OsIRT1 and OsIRT2 in rice. Soil Sci Plant Nut 52, 464-469.
[19] Satarug S, Garrett SH, Sens MA, Sens DA (2010). Cadmium, environmental exposure, and health outcomes. Environ Health Perspect 118, 182-190.
[20] Satoh-Nagasawa N, Mori M, Nakazawa N, Kawamoto T, Nagato Y, Sakurai K, Takahashi H, Watanabe A, Akagi H (2012). Mutations in rice (Oryza sativa) heavy metal ATPase 2 (OsHMA2) restrict the translocation of zinc and cadmium. Plant Cell Physiol 53, 213-224.
[21] Shi JC, Yu XL, Zhang MK, Lu SG, Wu WH, Wu JJ, Xu JM (2011). Potential risks of copper, zinc, and cadmium pollution due to pig manure application in a soil-rice system under intensive farming: a case study of Nanhu, China. J Environ Qual 40, 1695-1704.
[22] Singh KK, Ghosh S (2013). Regulation of glutamine synthetase isoforms in two differentially drought-tolerant rice (Oryza sativa L.) cultivars under water deficit conditions. Plant Cell Rep 32, 183-193.
[23] Takahashi A, Agrawal GK, Yamazaki M, Onosato K, Miyao A, Kawasaki T, Shimamoto K, Hirochika H (2007). Rice Pti1a negatively regulates RAR1-dependent defense responses. Plant Cell 19, 2940-2951.
[24] Ueno D, Kono I, Yokosho K, Ando T, Yano M, Ma JF (2009a). A major quantitative trait locus controlling cadmium translocation in rice (Oryza sativa). New Phytol 182, 644-653.
[25] Ueno D, Koyama E, Kono I, Ando T, Yano M, Ma JF (2009b). Identification of a novel major quantitative trait locus controlling distribution of Cd between roots and shoots in rice. Plant Cell Physiol 50, 2223-2233.
[26] Uraguchi S, Kamiya T, Sakamoto T, Kasai K, Sato Y, Nagamura Y, Yoshida A, Kyozuka J, Ishikawa S, Fujiwara T (2011). Low-affinity cation transporter (OsLCT1) regulates cadmium transport into rice grains. Proc Natl Acad Sci USA 108, 20959-20964.
[27] Wang FJ, Wang M, Liu ZB, Shi Y, Han TQ, Ye YY, Gong N, Sun JW, Zhu C (2015). Different responses of low grain-Cd-accumulating and high grain-Cd-accumulating rice cultivars to Cd stress. Plant Physiol Biochem 96, 261-269.
[28] Yu H, Wang JL, Fang W, Yuan JG, Yang ZY (2006). Cadmium accumulation in different rice cultivars and screening for pollution-safe cultivars of rice. Sci Total Environ 370, 302-309.
[29] Zhang XQ, Zhang GP, Guo LB, Wang HZ, Zeng DL, Dong GJ, Qian Q, Xue DW (2011). Identification of quantitative trait loci for Cd and Zn concentrations of brown rice grown in Cd-polluted soils. Euphytica 180, 173-179.
[30] Zong W, Tang N, Yang J, Peng L, Ma SQ, Xu Y, Li GL, Xiong LZ (2016). Feedback regulation of ABA signaling and biosynthesis by a bZIP transcription factor targets drought-resistance-related genes. Plant Physiol 171, 2810-2825.
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