Cloning of Wheat TaLCD Gene and Its Regulation on Osmotic Stress
Received date: 2019-06-14
Accepted date: 2019-11-05
Online published: 2019-11-14
Cysteine desulphydrase (CDes) can degrade cysteine (Cys) to form hydrogen sulfide (H2S). In this study, L-cysteine desulphydrase TaLCD from wheat (Triticum aestivum) was cloned and overexpressed in Arabidopsis thaliana. The effects of TaLCD on seed germination and root growth under osmotic stress in TaLCD overexpressing plants were examined, and the response to drought stress was also investigated. The results showed that, the seed germination rate of TaLCD overexpressing plants under salt treatment was remarkably higher than the wild type (WT), and the root length of TaLCD overexpressing plants was obviously longer when exposed to mannitol. Moreover, overexpression of TaLCD distinctly increased plant drought resistance. In addition, TaLCD overexpressing plants were more sensitive to ABA, with decreased seed germination rate and root length under ABA treatment. Furthermore, the expression of stress-responsive genes (COR47, RD29A, RAB18 and RD22) and ABA signaling pathway-related genes (NCED3, HAB1, HAB2, ABI1, ABI2 and ABF2) was significantly higher in TaLCD overexpressing plants than that in WT under drought stress. These results suggest that TaLCD enhances the drought and salt tolerance of plants possibly by ABA signaling pathway.
Key words： TaLCD; osmotic stress; ABA; germination and growth
Yang Zhang,Huajie Liu,Ruili Xue,Haixia Li,Hua Li . Cloning of Wheat TaLCD Gene and Its Regulation on Osmotic Stress[J]. Chinese Bulletin of Botany, 2020 , 55(2) : 137 -146 . DOI: 10.11983/CBB19109
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