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Chinese Bulletin of Botany >

2017 , Vol. 52 >Issue 3: 331 - 336

DOI: https://doi.org/10.11983/CBB16170

In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. ‘Variegata’

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  • Institute of Virus and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, China

# Co-first authors

Received date: 2016-08-16

  Accepted date: 2017-03-06

  Online published: 2017-05-27

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Abstract

We performed in vitro plantlet regeneration and multiplication of Haworthia retusa × cooperi cv. ‘Variegata’ for industrial production by taking the young alabastrum as explants. Calli were induced from explants on MS+5.0 mg∙L-1 6-BA+0.5 mg∙L-1 IBA medium. MS medium without 6-BA was found appropriate for the differentiation of calli. The regenerated shoots had the highest multiplication ratio and induction rate of chimeric bud (16.7 and 79.9%, respectively) when propagated on MS+0.2 mg∙L-1 6-BA. Our results show that the plantlet regeneration of H. retusa × cooperi cv. ‘Variegata’, through adventitious bud induction from callus was feasible and an appropriate concentration of cytokinin could improve the induction rate of chimeric buds. This study offers insight into the germplasm protection and industrial production of rare chimeric succulents.

Cite this article

Yan Wang, Haojie Mou, Yongping Lü, Haiying Li, Yiting Wang, Jianping Chen . In vitro Regeneration and Industrial Micropropagation of Haworthia retusa × cooperi cv. ‘Variegata’[J]. Chinese Bulletin of Botany, 2017 , 52(3) : 331 -336 . DOI: 10.11983/CBB16170

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