Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata
- College of Life Sciences, Jiangxi Normal University, Nanchang 330022, China
# Co-first authors
Received date: 2016-02-24
Accepted date: 2016-09-04
Online published: 2017-04-05
Abstract
We investigated the effects of amino acid supplements on thallus growth, superoxide dismutase (SOD) activity and huperzine-A accumulation in Huperzia serrata by adding aspartic acid, lysine, and tryptophan at different concentrations into the medium. With aspartic acid, the dry weight of the thallus increased by 18.50%-25.75% and the huperzine-A accumulation was significantly higher than in the control. The optimal concentration of aspartic acid was 0.5 mmol·L-1. The relative growth of thallus was significantly higher with 1 mmol·L-1 tryptophan than in the control, but the huperzine-A accumulation was slightly lower than for the control. The relative growth rate, dry weight and huperzine-A accumulation was lower for thallus cultured with lysine than the control. The SOD activity of the thallus cultured with aspartic acid was increased properly, and the huperzine-A accumulation of the thallus was increased. However, huperzine-A accumulation of the thallus was suppressed when SOD activity remained high in the long-term.
Cite this article
Man Chen, YishengTu, Linan Ye, Biyun Yang . Effect of Amino Acids on Thallus Growth and Huperzine-A Accumulation in Huperzia serrata[J]. Chinese Bulletin of Botany, 2017 , 52(2) : 218 -224 . DOI: 10.11983/CBB16033
References
| [1] | 陈永勤, 朱蔚华 (1997). 红豆杉属植物的组织、细胞及胚培养. 植物生理学通讯 33, 213-219. |
| [2] | 陈作毅, 张君诚 (2013). 石杉碱生物合成与代谢途径研究进展. 中华中医药杂志 28, 1815-1818. |
| [3] | 崔堂兵, 郭勇, 林炜铁 (2001). 提高植物细胞培养法生产次级代谢物产量的方法. 植物生理学通讯 37, 479-482. |
| [4] | 谷荣辉, 洪利亚, 龙春林 (2013). 植物细胞培养生产次生代谢物的途径. 植物生理学报 49, 869-881. |
| [5] | 吉枝单 (2015). 蛇足石杉离体叶状体生产石杉碱甲的工艺条件研究. 硕士论文. 南昌: 江西师范大学. pp. 13-34. |
| [6] | 吉枝单, 涂艺声, 丁明华, 陈雄, 蒋秀芳 (2014). 蛇足石杉离体培养产生有效成分的研究. 天然产物研究与开发 5, 645-649. |
| [7] | 简令成, 王红 (2009). 逆境植物细胞生物学. 北京: 科学出版社. pp. 137-153. |
| [8] | 马英姿, 刘江海, 许欢, 刘芬 (2015). 蛇足石杉的离体培养. 植物生理学报 51, 465-470. |
| [9] | 孟祥才, 王喜军 (2011). 活性氧促进道地药材质量形成的假说及其探讨. 中草药 42, 799-804. |
| [10] | 宋兴舜, 任静, 刘雪梅, 马双, 杨传平 (2009). 光合菌对黄瓜光合及抗氧化同工酶的影响. 植物学报 44, 587-593. |
| [11] | 苏文华, 张光飞, 李秀华 (2005). 植物药材次生代谢产物的积累与环境的关系. 中草药 36, 1415-1418. |
| [12] | 涂艺声, 丁明华 (2012). 产石杉碱甲的离体蛇足石杉叶状体诱导和增殖的培养方法. CN102550416A. 2012-07-11. |
| [13] | 王德立, 甘炳春, 齐耀东, 赵祥升 (2014). 蛇足石杉不同生长阶段植株中石杉碱甲含量的地区差异及生境影响的比较观察. 中国新药杂志 23, 326-332. |
| [14] | 王莉, 史玲玲, 张艳霞, 刘玉军 (2007). 植物次生代谢物途径及其研究进展. 植物科学学报 25, 500-508. |
| [15] | 吴荭, 庄平, 冯正波, 张超, 靳昌伟 (2005). 中国蛇足石杉资源调查与评估. 自然资源学报 20, 59-67. |
| [16] | 徐贤柱, 涂艺声, 吉枝单, 陈曼, 蔡险峰, 杨萍 (2015). 蛇足石杉离体培养物形态变化与石杉碱甲积累的研究. 植物学报 50, 733-738. |
| [17] | 翟雪霞, 李友勇 (2009). 几种氨基酸前体物对红豆杉愈伤组织的生长和紫杉醇含量的影响. 湖北农业科学 48, 2494-2496. |
| [18] | 郑书岩, 郁春辉, 沈征武 (2013). 石杉碱甲的合成研究进展. 有机化学 33, 2261-2270. |
| [19] | Beauchamp C, Fridovich I (1971). Superoxide dismutase: improved assays and an assay applicable to acrylamide gels.Anal Biochem 44, 276-287. |
| [20] | Hemscheidt T (2000). Tropane and Related Alkaloids. Biosynthesis. Vol. 209. Berlin: Springer. 175-206. |
| [21] | Shu S, Zhao X, Wang W, Zhang G, Cosoveanu A (2014). Identification of a novel endophytic fungus fromHuperzia serrata, which produces huperzine A. World J Microb Biot 30, 3101-3109. |
| [22] | Wu J, Ge X (2004). Oxidative burst, jasmonic acid biosynthesis, and taxol production induced by low-energy ultrasound inTaxus chinensis, cell suspension cultures. Biotechnol Bioeng 85, 714-721. |
| [23] | Zhang G, Wang W, Zhang X, Xia Q, Zhao X, Ahn Y (2015). De novo RNA sequencing and transcriptome analysis ofColletotrichum gloeosporioides es026 reveal genes related to biosynthesis of huperzine A. PLoS One 10, e0120809. |
| [24] | Zhu D, Wang J, Zeng Q, Zhang Z, Yan R (2010). A novel endophytic huperzine A-producing fungus,Shiraia sp. Slf14, isolated from Huperzia serrata. J Appl Microb 109, 1469-1478. |