We established the metabolomics method for investigating metabolites in Corydalis yanhusuo bulbs by gas chromatography-mass spectrometry. We also investigated the effect of different volumes of extracts and derived reagents (MSTFA) for peak signal intensity and numbers. We determined the best conditions with each 100 mg material extracted by use of 300 μL extract and 100 μL MSTFA. To detect samples of C. yanhusuo tuber, we obtained 92 kinds of compounds, which contained 5 fatty acids, 21 organic acids, 16 amino acids, 6 sugars, 23 other ingredients, and 21 unknown compounds. We also studied the pattern recognition of C. yanhusuo tuber metabolites by principal component analysis. We could provide valid data and reference values for further research into C. yanhusuo tubers.
Pengfei Wang, Qianqian Wang, Xianen Li, Minjian Qin
. Metabolites Research of Corydalis yanhusuo Tubers with Gas Chromatography-mass Spectrometry[J]. Chinese Bulletin of Botany, 2012
, 47(2)
: 149
-154
.
DOI: 10.3724/SP.J.1259.2012.00149
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