Plants have several strategies to adapt to different environments. Lonicera confusa is a typical species in karst calcium (Ca)-rich environments; to adapt to the Ca-rich environment, it excretes calcium by leaf stoma. In the present study, the calcium-excretion process of L. confusa was investigated by scanning electron microscopy and energy-dispersive X-ray spectroscopy (EDX). We compared the different types of Ca crystals by self-assembly of secreted products with point and line-by-line scanning. Ca crystals are formed after Ca salt is excreted by stoma of L. confusa in the karst Ca-rich environment. Ca salt could self-assemble 3 types of crystals, prisms, ball and crystal sand. Crystals have a large amount of Ca, and sulfur (S) and other elements, which are present in certain ratio. The crystal nucleus protein is rich in S element, which is beneficial for biomineralization of Ca-related crystal and others. EDX could be effectively used for microprobe analysis of plant leaves and identifying plant-excreted products.
Geng Wu, Chunhua Fu, Yongwei Huang, Wei Li, Longjiang Yu, Maoteng Li
. Calcium Salt Excreted by Stoma and Its Biomineralization in Lonicera confusa Under a Calcium-rich Environment[J]. Chinese Bulletin of Botany, 2011
, 46(6)
: 658
-664
.
DOI: 10.3724/SP.J.1259.2011.00658
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