Rice is one of the most important cereal crops. The introduction of dwarf rice has led to the first “green revolution”. However, the genetic uniformity of dwarf gene in rice breeding is a concern, which severely affects the continuous increase in rice harvest. In the present study, we mutated the indica cultivar M804 by using 60Coγ-Ray and obtained a genetically stable rice dwarf mutant (MU101), which was crossed with a japonica cultivar Taigeng 16 to investigate the genetic mode and map the gene. Genetic analysis revealed the phenotype of the dwarf mutant, which we designated tentatively as ds1, controlled by a pair of recessive genes. Polymorphic analysis of simple sequence repeat markers demonstrated the DS1 gene located on chromosome 5. With a larger mapping population and more Indel markers, we further mapped the DS1 gene between 2 Indel markers with a physical region of about 384 kb. These results provide a basis for molecular cloning and production application of DS1.
Dewei Yang, Meijuan Zeng, Libin Lu, Ning Ye, Chengde Liu, Xianghua Zheng, Xinfu Ye
. Genetic Analysis and Mapping of Rice Dwarf Mutant ds1[J]. Chinese Bulletin of Botany, 2011
, 46(6)
: 617
-624
.
DOI: 10.3724/SP.J.1259.2011.00617
郭龙彪，储成才，钱前 (2006)．水稻突变体与功能基因组学．植物学通报 23，1-13．
胡江 (2006)．一个新水稻矮化突变体d63的遗传分析与分子定位．扬州：扬州大学．
胡兴明 (2005)．水稻矮秆DWARF1-Like基因的精细定位．北京：中国农业科学院．
刘仁虎，孟金陵 (2003)．MapDraw，在Excel中绘制遗传连锁图的宏．遗传 25，317-321．
夏令，陈亮，郭迟鸣，张红心，赵政，沈明山，陈亮 (2007)．一个新的水稻矮秆突变体sd-sl的遗传与基因定位研究．厦门大学学报(自然科学版) 46，847-851．
杨德卫，卢礼斌，郑向华，黄镜浩，叶宁，刘成德，程朝平，何琴，叶新福 (2011)．一个水稻矮秆突变体的诱发及鉴定研究．核农学报 25，第2期出版．
于永红，斯华敏 (2005)．水稻矮化相关基因的研究进展．植物遗传资源学报，6 334-347．
章忠贵，刘斌美，许学，张丽丽，王敏，吴跃进 (2010)．水稻株高突变系的农艺性状与抗倒伏研．核农学报 24，430-435．
AriteT, Iwata H, Ohshima K, Maekawa M, Nakajima M, Kojina M, Sakakibara H, Kyozuka J (2007). DWARF10, an RMS1/MAX4/DAD1 ortholog, controls lateral bud outgrowth in rice. Plant J 51, 1019-1029.
Ashikari M, Wu JZ, Yano M, Sasaki T, Yoshimura A (1999). Rice gibberellin-insensitive dwarf mutant gene Dwarf1 encodes the α-subunit of GTP-binding protein. PNAS 96,10284-10289.
Hong Z, Ueguchi-Tanaka M, Umemura K, Uozu S, Fujioka S, Takatsuto S, Yoshida S, Ashikari M, Kitano H, Matsuoka M (2003) . A rice brassinosteroid-deficient mutant, ebisu dwarf (d2), is caused by a loss of function of a new member of cytochrome P450. The Plant Cell 15, 2900-2910.
Ishikawa S, Maekawa M, Arite T, Onishi K, Takamure I, Kyozuka J (2005).Suppression of tiller bud activity in tillering dwarf mutants of rice. Plant Cell Physiology 46, 79-86.
Itoh H, Ueguchi-Tanaka M, Sentoku N, Kitano H, Matsuka M, Kobayashi M (2001). Cloning and functional analysis of gibberellin 3?-hydroxylase genes that are differently expressed during the growth of rice. PNAS 98, 8909-8914.
Lander ES, Green P, Abrahamson J, Barlow A, Daly MJ, Lincoln SE, Newberg LA (1987). Corrigendum to “Mapmaker: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations”. Genomics 1, 174-181.
Li XL, Wu C, Deng XJ, Wang PR, Li RD, Yang ZR (2003). Molecular tagging and effect analysis of a new small grain dwarf gene in rice. Acta Botanica Sinica 45, 757-761.
Murray MG, Thompson WF (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 819, 4321-4325.
Nishikubo N, Araki T, Kajita S, Kuroda K, Kitano H, Katayama Y (2000). Specific accumulation of polysaccharide-linked hydroxycinnamoyl esters in the cell walls of irregularly shaped and collapsed internode parenchyma cells of the dwarf rice mutant Fukei 71. Plant Cell Physiol 41, 776-784.
Qin RZ, Qiu Y, Cheng ZJ, Shan XY, Guo XP, Zhai HQ, Wan JM (2008). Genetic analysis of a novel dominant rice dwarf mutant 986083D. Euphytica 160, 379-387.
Quarrie S, Lazic-Jancic V, Kovacevic D, Steed A, Pekic S (1999). Bulk segregant analysis with molecular markers and its use for improving drought resistance in maize. J Exp Bot 50, 1299–1306.
Sasaki A, Itoh H, Gomi K, Ueguchi-Tanaka M, Ishiyama K, Kobayashi M, Jeong DH, An G, Kitano H, Ashikari M,Matsuoka M (2003). Accumulation of the phosphorylated repressor for GA signaling in an F-box mutant. Science 299, 1896-1898.
Spielmeyer W, Ellis MH, Chandler PM (2002). Semidwarf (sd-1), “green revolution” rice, contains a defective gibberellin 20-oxidase gene. Proc Natl Acad Sci USA 99, 9043-9048.
Tanabe S, Ashikari M, Fujioka S, Takatsuto S, Yoshida S, Yano M, Yoshimura A, Kitano H, Matsuoka M, Fujisawa Y, Kata H, Iwasaki Y (2005). A novel cytochrome p450 is implicated in Brassinosteroid biosynthesis via the characterization of a rice dwarf mutant, dwarf11, with reduced seed length. The Plant Cell 17, 776-790.
Ueguchi-Tanaka M, Ashikari M, Nakajima M, Itoh H, Katoh E, Kobayashi M, Chow TY, Hsing YI, Kitano H, Yamaguchi I, Matsuoka M (2005). GIBBERELLIN INSENSITIVE DWARFl encodes a soluble receptor for gibberellin. Nature 437, 693-698.
Yuan LP (2000).Super hybrid rice, Chinese Rice Research Newsletter 8, 13-l5 (Chinese Journal in English).
Zou JH, Zhang SY, Zhang W P, Li G, Chen ZX, Zhai WX, Zhao XF, Pan XB, Xie Q, Zhu LH (2006). The rice HIGH-TILLERING DWARF1 encoding an ortholog of Arabidopsis MAX3 is required for negative regulation of the outgrowth of axillary buds. The Plant Journal 48, 687-696.
Home