Chinese Bulletin of Botany >

2015 , Vol. 50 >Issue 4: 495 - 503

DOI: https://doi.org/10.11983/CBB15038

TECHNIQUE AND METHOD

Using 3D-SIM Structure Illumination Microscope to Localize Proteins in Plant Subcellular Compartments

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  • 1College of Life Sciences, Jilin Agricultural University, Changchun 130118, China
    2Institute of Agricultural Biotechnology, Jilin Academy of Agricultural Sciences, Changchun 130033, China
    3Institute of Frontier Medical Sciences, Jilin University, Changchun 130021, China
    4College of Life Sciences, Harbin Normal University, Harbin 150080, China

? These authors contributed equally to this paper

Received date: 2015-02-16

  Accepted date: 2015-03-30

  Online published: 2015-05-07

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Abstract

The information on protein subcellular localization is important to elucidate protein function, and imaging technology is one of the important approaches to visualize protein localization. However, conventional microscopy techniques can barely resolve details of subcellular structures mainly because of the auto-fluorescence interference with their limited imaging resolution. In recent years, super-resolution optical imaging technologies have been successfully used in human and animal cell research for their 8-fold higher spacial resolution over laser confocal microscopy. Application of these technologies in plant cells has not been reported, probably due to the peculiarity of plant cells. Here, we report the successful use of DeltaVision OMX microscope technology for visualizing Zea mays sucrose synthase 1 (ZmSUS-SH1) around chloroplast grana in transgenic tobacco epidermal cells. OMX microscopy can overcome the cellular chlorophyll fluorescence interference in imaging fluorescent fusion proteins. We also developed an optimized sample preparation protocol for using DeltaVision OMX microscope technology with plant materials.

Cite this article

Yue Liu, Yuejia Yin, Chongyang Liang, Dianshuai Huang, Yang Wang, Yanzhi Liu, Yao Dou, Shudan Feng, Dongyun Hao . Using 3D-SIM Structure Illumination Microscope to Localize Proteins in Plant Subcellular Compartments[J]. Chinese Bulletin of Botany, 2015 , 50(4) : 495 -503 . DOI: 10.11983/CBB15038

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