We used the leaves and stems of aseptic seedlings of 5 rose cultivars that can successfully live outside through the winter in Heilongjiang Province as explants to investigate callus induction and plant regeneration. The leaves and stems of the 5 rose cultivars could induce callus; media containing 2,4-D could induce callus better; and a high concentration of cytokinin was not suitable for callus induction. TDZ played an important role in callus differentiation; culture under light conditions was effective for callus differentiation; and callus differentiation decreased with increasing number of subcultures. Plant regeneration could be successfully induced from the callus of 2004-8 and 2004-9 in experimentation, and the callus differentiation rate of the 2 varieties was 45% and 38%, respectively.
Xue Chen, Jinzhu Zhang, Bingbing Pan, Chengjin Sang, Xue Ma, Tao Yang, Daidi Che
. Callus Induction and Plant Regeneration of Rose[J]. Chinese Bulletin of Botany, 2011
, 46(5)
: 569
-574
.
DOI: 10.3724/SP.J.1259.2011.00569
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