[an error occurred while processing this directive] [an error occurred while processing this directive] [an error occurred while processing this directive]
[an error occurred while processing this directive]

Chinese Bulletin of Botany >

2011 , Vol. 46 >Issue 4: 470 - 475

DOI: https://doi.org/10.3724/SP.J.1259.2011.00470

Identification of 9-oxo-10,11-dehydroagerophorone in Eupatorium adenophorum by High Performance Liquid Chromatography

Expand
  • 1Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China

    2Key Laboratory of Plateau and Subtropical Crops, Xichang College, Xichang 615000, China

Received date: 2010-09-15

  Revised date: 2011-02-12

  Online published: 2011-07-01

Fold

Abstract

We isolated and purified the component of 9-oxo-10,11-dehydroagerophorone (Euptox A), a hepatotoxin and an insecticide constituent, from Eupatorium adenophorum leaves by silica gel column chromatography and reverse-phase high performance liquid chromatography (HPLC). We developed an HPLC method for determining Euptox A from E. adenophorum, which was confirmed by HPLC-DAD-MS. The chromatographic separation was achieved on a Cosmosil Rp C18 column with methanol:water (60:40, v/v) as the mobile phase, flow rate 0.8 mL·min–1 and wavelength 255 nm. Data were corroborated by HPLC-MS with electro-spray ionization of positive ion mode. The limit of detection for Euptox A was 0.4 μg·g–1. The recoveries were 97.3%–103.7% and relative standard deviations 2.4%–3.5%. Euptox A was mainly distributed in leaves (1.34–8.41 mg·g–1) and only a small amount was detected in flowers, stems, or roots (< 0.06 mg·g–1). Euptox A mainly accumulated in plant leaves in the vegetative period (from May to October), with less accumulation in the reproductive period (from November to the next April). The established HPLC method is simple, quick and could be used for component determination in E. adenophorum products for quality control and safety evaluation.

Cite this article

Jie Bai, Aocheng Cao, Meixia Guo, Xiaoyan Liu, Xiaowen Liu, Hai Liang, Bo Zhou . Identification of 9-oxo-10,11-dehydroagerophorone in Eupatorium adenophorum by High Performance Liquid Chromatography[J]. Chinese Bulletin of Botany, 2011 , 46(4) : 470 -475 . DOI: 10.3724/SP.J.1259.2011.00470

[an error occurred while processing this directive]

References

曹坳程，芮昌辉，郭美霞，袁会珠，杨代斌，陈福良（2006）. 紫茎泽兰的化学防除及生态修复效果安全与环境学报，，6（3）：80~85
周自玮，段新慧，徐驰，奎嘉祥（2006）. 紫茎泽兰饲喂山羊试验研究,饲料工业27（19）： 25~28
Bohlman F, Gupta P K(1981). Six cadinane derivatives from Ageratina adenophora. Phytochemistry, 20:1432~1433
He L, Hou J, Gan M L, SHi JG, Chantrapromma S, Fun HK, Williams L D, SUNG H Y (2008). Journal of Natural Products, 71:1485-1488
He L, Yang J, Cao AC，Liu YM， An Y, Shi JG(2006). A new Sequiterpenoid from Eupatorium adenophorum Spreng. Chinese Journal of Chemistry. 24, 1375-1377
Kaushal V, Dawra R K, Sharma O P, Kurade N P(2001). Biochemical alterations in the blood plasma of rats associated with hepatotoxicity induced by Eupatorium adenophorum.Veterinary Research Communications, 2001, 25: 601~608 Bohlman F, Gupta P K. Phytochemistry， 20：1432-1433
Oelrichs PB, Calanasan CA, Madleod J K, Seawright AA, Ng JC(1995). Isolation of a compound from Eupatorium adenophorum (spreng.) [Ageratina adenophora (spreng.)] causing hepototoxicity in mice.Natural Toxins. 3: 350~354
Sun XY, Lu ZH, Sang WG(2004). Review on studies of Eupatorium adenophorum-an important invasive species in China.Journal of Forestry Research, 15:319~322
Verma A, Yadava BPS, Sampath KT. Ind. J（1987）.Possible use of Eupatorium adenophorum Spreng. in animal feeding. Anim. Nutr. , 4:189~192
Options
Outlines

/

〈 〉

[an error occurred while processing this directive]