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Chinese Bulletin of Botany >

2016 , Vol. 51 >Issue 2: 251 - 256

DOI: https://doi.org/10.11983/CBB15036

TECHNIQUE AND METHOD

Tissue Culture of the Succulent Plant Sedum clavatum

  • Fang Liu ,
  • Yinghong Tang ,
  • Youmei Yuan ,
  • Qingquan Guo ,
  • Fan Shen ,
  • Jianrong Chen
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  • 1Department of Biological and Environmental Engineering of Changsha University, Changsha 410022, China
    2Agriculture College of Hunan Agriculture University, Changsha 410128, China
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†These authors contributed equally to this paper

Received date: 2015-02-16

  Accepted date: 2015-05-11

  Online published: 2016-03-31

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Abstract

The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L-1 6-BA+0.1 mg·L-1 NAA+1.0 mg·L-1 KT; for redifferentiation 3/4MS+3.0 mg·L-1 6-BA+0.3mg·L-1 NAA; and for rooting 1/2MS+0.03 mg·L-1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.

Key words： Sedum clavatum; callus induction; plant regeneration

Cite this article

Fang Liu , Yinghong Tang , Youmei Yuan , Qingquan Guo , Fan Shen , Jianrong Chen . Tissue Culture of the Succulent Plant Sedum clavatum[J]. Chinese Bulletin of Botany, 2016 , 51(2) : 251 -256 . DOI: 10.11983/CBB15036

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