Tissue Culture of the Succulent Plant Sedum clavatum
- 1Department of Biological and Environmental Engineering of Changsha University, Changsha 410022, China
2Agriculture College of Hunan Agriculture University, Changsha 410128, China
Received date: 2015-02-16
Revised date: 2015-05-06
Online published: 2016-03-31
Supported by
湖南省科技计划(No.2014CK4018)
Abstract
The leaf of Sedum clavatum was used to induce callus; through callus induction, bud induction, rooting and transplatation, aseptic-frequency regeneration systems were established. The optimal medium for induction was MS+3.0 mg·L–1 6-BA+0.1 mg·L–1 NAA+1.0 mg·L–1 KT; for redifferentiation 3/4MS+3.0 mg·L–1 6-BA+0.3mg·L–1 NAA; and for rooting 1/2MS+0.03 mg·L–1 NAA. The callus induction, redifferentiation and rooting rate was 95.7%, 80% and 94.89%, respectively. Using perlite:vermiculite (1:2) as a matrix, the transplant survival rate was >90%. We have established a high-frequency regeneration system for S. clavatum.
Key words： Sedum clavatum; callus induction; plant regeneration
Cite this article
Fang Liu, Yinghong Tang, Youmei Yuan, Qingquan Guo, Fan Shen, Jianrong Chen . Tissue Culture of the Succulent Plant Sedum clavatum[J]. Chinese Bulletin of Botany, 2016 , 51(2) : 251 -256 . DOI: 10.11983/CBB15036