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DOI: https://doi.org/10.11983/CBB24118

Analysis of physicochemical characteristics and expression characteristics of lagerstroemia GolS family genes

  • XU Tian-Tian ,
  • CAO Yi ,
  • YANG Pei-Jian ,
  • YANG Fan ,
  • ZHOU Xiao-Qian ,
  • CHEN Yan-Hong ,
  • WEI Hui ,
  • ZHANG Jian
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  • 1School of Life Sciences, Nantong University, Nantong 226019, China; 2Nantong Key Laboratory of Ornamental Plant Genetics and Breeding, Nantong 226019, China

Received date: 2024-08-04

  Revised date: 2024-11-12

  Online published: 2024-11-26

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Abstract

galactinol synthase (GolS) is a key enzyme in the biosynthesis pathway of raffinose family oligosaccharides (RFOs), providing activated galactosyl for raffinose family oligosaccharides. Regulation of biosynthesis and accumulation of raffinose oligosaccharides in plants plays an important role in plant response to abiotic stress. However, the molecular structure characteristics of GolS gene family members of Lagerstroemia indica have not been reported. In this study, 13 members of the LiGolS gene family were identified at the whole genome level, and their physicochemical properties, chromosome localization, evolutionary relationship, gene structure, conserved motifs, and expression under salt stress were analyzed. The results showed that the 13 LiGolS genes were unevenly distributed on 10 chromosomes. The isoelectric point of the 13 LiGolS proteins was 4.75−9.45, the molecular weight was 37.69−46.12 kDa, and the amino acid number was 327−404 aa. Subcellular localization prediction results showed that 9 proteins were located in the nucleus, 6 proteins were located in the chloroplast, 1 protein was located in the mitochondria, 5 proteins were located in the cytoplasm, and 1 protein was located in the vacuole. Thirteen gene members contain exons with a number of 0–4. Analysis of LiGolS expression based on salt stress showed that all LiGolS gene members showed varying degrees of up-regulated expression after salt treatment, indicating that these genes may be involved in the salt stress response of Crape Myrtle. The results of this study laid a foundation for further functional analysis of LiGolS gene.

Key words： Crape Myrtle; GolS gene family; Salt stress

Cite this article

XU Tian-Tian , CAO Yi , YANG Pei-Jian , YANG Fan , ZHOU Xiao-Qian , CHEN Yan-Hong , WEI Hui , ZHANG Jian . Analysis of physicochemical characteristics and expression characteristics of lagerstroemia GolS family genes[J]. Chinese Bulletin of Botany, 0 : 1 -0 . DOI: 10.11983/CBB24118

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