不同熟期玉米叶片衰老特性及其对叶际细菌的影响

杨文丽; 李钊; 刘志铭; 张志华; 杨今胜; 吕艳杰; 王永军

doi:10.11983/CBB24037

植物学报 >

2024 , Vol. 59 >Issue 6: 0 - 0

DOI: https://doi.org/10.11983/CBB24037

研究论文

不同熟期玉米叶片衰老特性及其对叶际细菌的影响

杨文丽 ,
李钊 ,
刘志铭 ,
张志华 ,
杨今胜 ,
吕艳杰 ,
王永军

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¹吉林农业大学农学院, 长春 130118; ²吉林省农业科学院农业资源与环境研究所/农业农村部作物生理生态与耕作重点实验室, 长春 130033; ³吉林大学植物科学学院/吉林省植物遗传改良工程实验室, 长春 130062

收稿日期: 2024-03-09

修回日期: 2024-05-07

网络出版日期: 2024-06-11

基金资助

吉林省科技发展计划项目(No.20220508096RC)、国家重点研发计划项目(No.2023YFD2301703)、现代农业产业技术体系项目(No.CARS-02-19)和国家自然科学基金项目(No.U23A6001-01)

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Senescence Characteristics of Maize Leaves at Different Maturity Stages and Their Effect on Interleaf Bacteria

YANG Wen-Li ,
LI Zhao ,
LIU Zhi-Ming ,
ZHANG Zhi-Hua ,
YANG Jin-Qing ,
LV Yan-Jie ,
YU Yong-Jun

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¹College of Agronomy, Jilin Agricultural University, Changchun 130118, China; ²Institute of Agricultural Resources and Environment, Jilin Academy of Agricultural Sciences/Key Laboratory of Crop Physiology, Ecology and Tillage, Ministry of Agriculture and Rural Affairs, Changchun 130033, China; ³School of Plant Science, Jilin University/Jilin Plant Genetic Improvement Engineering Laboratory, Changchun 130062, China

Received date: 2024-03-09

Revised date: 2024-05-07

Online published: 2024-06-11

Supported by

Jilin Province Key Technology R&D Program; National Key Research and Development Program (NKRDP) projects; National Natural Science Foundation of China (NSFC); Modern Agricultural Industrial Technology System Program

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摘要

叶片作为作物光合器官, 其衰老进程对产量形成具有重要影响, 但叶片衰老与叶际微生物间的关系研究较少。为探讨玉米叶片衰老过程对叶际细菌群落的影响, 本研究以东北春玉米区3个不同熟期玉米品种(早熟品种黑科玉17, H17、中熟品种中单111, Z111、和晚熟品种沈玉21, S21)为试验材料, 从早熟品种开花期开始对3个玉米品种穗位叶进行5次取样, 测定衰老生理指标, 同时基于高通量测序技术测定叶际内源和外源细菌的群落组成。结果表明, 在生育后期, 中熟和晚熟品种叶含水率、POD和SOD活性显著高于早熟品种。在门水平, 蓝菌门(Cyanobacteria)是中熟和晚熟的特有菌门; 在属水平, 玉米叶片内外源共有细菌鞘氨醇单胞菌属(Sphingomonas)、甲基杆菌属(Methylobacterium)、异常球菌属(Deinococcus)相对丰度在IV和V时期显著降低, 而内源细菌链霉菌属(Streptomyces)和外源细菌P3OB-42属则在衰老后期显著富集, 三个品种变化趋势相似, 相对丰度差异显著。内外源细菌相对丰度存在显著差异, 前5位的外源细菌占60%以上, 而对内源细菌而言, 前5位仅占30%以上。可溶性糖含量、光合色素含量和SOD活性与细菌群落结构和丰富度显著相关。综上, 中熟和晚熟品种能有效延长叶片持绿期, 维持后期叶片生理活性, 延缓衰老。衰老对内源细菌群落组成和多样性的影响显著大于外源细菌, 不同熟期品种间存在显著分异的菌属, 且可溶性糖含量、光合色素含量和SOD活性是影响叶际细菌群落以及优势物种的关键因子。

关键词： 玉米; 熟期; 叶片衰老; 叶际细菌; 群落组成

本文引用格式

杨文丽 , 李钊 , 刘志铭 , 张志华 , 杨今胜 , 吕艳杰 , 王永军 . 不同熟期玉米叶片衰老特性及其对叶际细菌的影响[J]. 植物学报, 2024 , 59(6) : 0 -0 . DOI: 10.11983/CBB24037

Abstract

As a photosynthetic organ of crops, the senescence process of leaves has an important impact on yield formation, but the relationship between leaf senescence and interleaf microorganisms has been less studied. In order to explore the influence of the senescence process of maize leaves on the interleaf bacterial community, this study took three maize varieties of different maturity (early maturity variety Heike Yu 17, H17, medium maturity variety Zhongdan 111, Z111, and late maturity variety Shen Yu 21, S21) in the spring maize area of Northeast China as the experimental materials, and the leaves of the ear position of the three maize varieties were sampled five times starting from the blooming stage of early maturity varieties, and the physiological indexes of senescence were determined, and at the same time The community composition of endogenous and exogenous bacteria in the interleaf was determined based on high-throughput sequencing technology. The results showed that at the late reproductive stage, leaf water content, POD and SOD activities were significantly higher in the mid- and late-maturing varieties than in the early-maturing varieties. At the phylum level, Cyanobacteria were endemic to medium- and late-maturing cultivars; at the genus level, the relative abundance of endogenous shared bacteria Sphingomonas, Methylobacterium, and Deinococcus in maize leaves was significantly reduced during IV and V periods The relative abundance of endogenous bacteria Streptomyces and exogenous bacteria P3OB-42 were significantly enriched in the late senescence period, with similar trends and significant differences in relative abundance among the three species. The relative abundance of endogenous and exogenous bacteria differed significantly, with the top 5 exogenous bacteria accounting for more than 60%, while for endogenous bacteria, the top 5 accounted for only more than 30%. Soluble sugar content, photosynthetic pigment content and SOD activity were significantly correlated with bacterial community structure and abundance. In conclusion, medium- and late-maturing varieties were effective in prolonging leaf greening period, maintaining late leaf physiological activity and delaying senescence. The effects of senescence on the composition and diversity of endogenous bacterial communities were significantly greater than those of exogenous bacteria, and there were significantly differentiated genera among varieties of different maturity stages. Moreover, soluble sugar content, photosynthetic pigment content and SOD activity were the key factors affecting the interleaf bacterial communities as well as the dominant species.

Key words： corn; ripening stage; leaf senescence; interleaf microbes; community composition

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