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DOI: https://doi.org/10.11983/CBB24047

研究报告

基于高密度Bin图谱的水稻苗期耐热性QTL定位

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  • 1江苏省农业科学院粮食作物研究所, 国家水稻改良中心南京分中心, 国家耐盐碱水稻技术创新中心华东中心, 江苏省优质水稻工程技术研究中心, 南京 210014; 2江苏省农业科学院无锡分院生物育种研究室, 无锡 214000

收稿日期: 2024-03-28

  修回日期: 2024-05-20

  网络出版日期: 2024-05-30

基金资助

国家产业技术体系建设专项(No. CARS-01)和江苏省种业振兴“揭榜挂帅”项目(No. JBGS(2021)040)

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Mapping of QTL for Heat Tolerance at Seedling Stage in Rice Based on a High-Density Bin Map

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  • 1Institute of Food Crops, Jiangsu Academy of Agricultural Sciences; Nanjing Branch of China National Center for Rice Improvement; East China Branch of National Center of Technology Innovation for Saline-Alkali Tolerant Rice; Jiangsu High Quality Rice Research & Development Center, Nanjing 210014, China; 2Biological Breeding Research Laboratory, Wuxi Branch of Jiangsu Academy of Agricultural Sciences, Wuxi 214000, China

Received date: 2024-03-28

  Revised date: 2024-05-20

  Online published: 2024-05-30

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摘要

鉴定控制水稻(Oryza sativa)高温耐性的新位点和候选基因, 为耐热遗传育种提供支撑,具有重要的理论和实践意义。利用粳稻(O. sativa subsp. japonica)品种TD70和籼稻(O. sativa subsp. indica)品种Kasalath衍生的重组自交系群体(RILs)为研究材料, 构建了基于深度重测序的高密度Bin遗传图谱, 以幼苗存活率为指标, 使用QTL IciMappingv软件完备复合区间作图法对控制水稻苗期高温胁迫下的幼苗存活率进行QTLs分析。共检测到26个控制苗期耐热性QTLs, 分布在除第3号染色体外的11条染色体上, LOD值为2.59–16.15, 其中4个QTLs的LOD值大于10, 7个QTLs和已知高温耐性QTLs的位置存在重叠或者部分重叠, 其中主效QTL位点qHTSR5.1位于第5号染色体20.41–20.48 Mb区间, LOD值为12.07, 解释4.86%的表型贡献率。对4个主效QTLs区间进行基因功能注释和亲本间序列分析, 共发现27个注释有功能、在2个亲本间编码区存在非同义突变的基因。根据候选基因SNP的类型对RILs群体家系进行基因等位型分类和效应分析, 发现5个基因其不同等位型的RILs家系在高温处理后的幼苗存活率上存在显著差异, 推测可能为候选基因, 可用于后续水稻高温耐性的分子机理研究。

关键词： 耐热性; 高密度Bin图谱; QTL定位; 水稻; 苗期

本文引用格式

赵凌, 管菊, 梁文化, 张勇, 路凯, 赵春芳, 李余生, 张亚东 . 基于高密度Bin图谱的水稻苗期耐热性QTL定位[J]. 植物学报, 0 : 0 -0 . DOI: 10.11983/CBB24047

Abstract

Identification of new loci and genes related to heat tolerance is very important for the genetic mechanism research and breeding of new heat-tolerant rice varieties. A recombinant inbred lines (RILs) was developed by crossing the japonica rice TD70 and the indica rice Kasalath. The high-density genetic linkage map with recombination Bin markers was constructed based on the re-sequencing. Based on the genotype and phenotype data of the RILs, QTL mapping of the high temperature seedling survival rate (HTSR) was performed by ICIM method of the QTL Ici Mapping software. 26 QTLs related to HTSR of rice were detected, distributed on 12 chromosomes except 3. The LOD value of single QTL ranged from 2.59–16.15, respectively. Among of them, four QTLs were detected with LOD values greater than 10. Seven QTLs were found to locate in the same interval or adjacent to known heat tolerance QTLs. Major locus qHTSR5.1 located in the 20.41-20.48 Mb of Chr. 5 with LOD value 12.07, which explained 4.86% of the total phenotypic variation for HTSR. According to the annotation and sequences analysis of genes located in the region of four major QTLs, we found that twenty-seven annotated genes with non-synonymous mutations in the coding regions between TD70 and Kasalath. The HTSR of RILs had significant difference between the RILs with different haplotype of five genes, indicating that they might be the candidate genes for HTSR. 

Key words： heat tolerance; high-density bin map; QTL mapping; rice; seedling stage

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